CD3E/CD3 epsilon [Biotin], His, Human
CD3E/CD3 Epsilon [Biotin], His, Human

Immobilized Anti-CD3 Antibody, hFc Tag at 1 μg/ml (100 μL/well) on the plate. Dose response curve for CD3E/CD3 epsilon [Biotin], His, Human, His Tag with the EC50 of 1.0 ng/ml determined by ELISA.

CD3E/CD3 Epsilon [Biotin], His, Human

CD3E/CD3 epsilon [Biotin], His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD3E/CD3 epsilon [Biotin], His, Human

CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Z04009
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Product Introduction
Species Human
Protein Construction
CD3E/CD3 epsilon (Asp23-Asp126)
Accession # P07766		 His
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Anti-CD3 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate can bind CD3E/CD3 epsilon[Biotin], His, Human. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 12.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 14-18 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS，(pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • CD3E/CD3 Epsilon [Biotin], His, Human
    • CD3E/CD3 Epsilon [Biotin], His, Human

    Immobilized Anti-CD3 Antibody, hFc Tag at 1 μg/ml (100 μL/well) on the plate. Dose response curve for CD3E/CD3 epsilon [Biotin], His, Human, His Tag with the EC50 of 1.0 ng/ml determined by ELISA.

  • CD3E/CD3 Epsilon [Biotin], His, Human
    • CD3E/CD3 Epsilon [Biotin], His, Human

    CD3E/CD3 epsilon [Biotin], His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Synonyms CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.