Immobilized CD3E&CD3G hFc Chimera [Biotin], Avi, Human, hFc Tag at 1 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, mFc Tag with the EC50 of 35.1 ng/ml determined by ELISA.

The purity of CD3E&CD3G hFc Chimera [Biotin], Avi, Human is greater than 95% as determined by SEC-HPLC.

CD3E&CD3G hFc Chimera [Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Anti-CD3 Anitibody, hFc Tag at 5 μg/ml(100 μl/well) on the plate. Dose response curve for CD3E&CD3G hFc Chimera [Biotin], Avi, Human, hFc Avi Tag with the EC50 of 0.20 μg/ml determined by ELISA.

Anti-CD3 Antibody, hFc Tag immobilized on CM5 Chip can bind CD3E&CD3G hFc Chimera [Biotin], Avi, Human, hFc-Avi Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).

CD3E&CD3G hFc Chimera [Biotin], Avi, Human

T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E&CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Cat. No.	Z03994
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
Price	¥139,907.00


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Product Introduction

Species

Human

Protein Construction

CD3E & CD3G [Asp23-Asp126 (CD3E) & Gln23-Ser116 (CD3G)] Accession # P07766 (CD3E) & P09693 (CD3G)	hFc	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized CD3E&CD3G hFc Chimera [Biotin], Avi, Human at 1μg/ml (100μl/well) on streptavidin (5μg/ml) precoated plate can bind OKT3, mFc Tag. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

40 kDa (CD3E) and 36.5 kDa (CD3G)

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from a 0.22 μm filtered solution in PBS，(pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples

The purity of CD3E&CD3G hFc Chimera [Biotin], Avi, Human is greater than 95% as determined by SEC-HPLC.

CD3E&CD3G hFc Chimera [Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Anti-CD3 Antibody, hFc Tag immobilized on CM5 Chip can bind CD3E&CD3G hFc Chimera [Biotin], Avi, Human, hFc-Avi Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).

Background

Target Background

Synonyms

CD3; CD3G; CD3g; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

CD3E&CD3G hFc Chimera [Biotin], Avi, Human

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