CD3E&CD3D [Biotin], His, Human

CD3E&CD3D [Biotin], His, Human

T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Z03992
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Product Introduction
Species Human
Protein Construction
CD3E & CD3D [Asp23-Asp126 (CD3E) & Phe22-Ala105 (CD3D)]
Accession # P07766 (CD3E) & P04234 (CD3D)		 His
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD3E&CD3D [Biotin], His, Human at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5 μg/ml) can bind OKT3, mFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 17.9 kDa (CD3E) and 16.7 kDa (CD3D)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS，(pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Background
Target Background T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Synonyms CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.