CD3E/CD3 epsilon hFc Chimera, Human
CD3E/CD3 Epsilon HFc Chimera, Human

Immobilized CD3E/CD3 epsilon hFc Chimera, Human, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 58.3 ng/ml determined by ELISA.

CD3E/CD3 Epsilon HFc Chimera, Human

CD3E/CD3 epsilon hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD3E/CD3 epsilon hFc Chimera, Human

T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E&CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Z03991
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Product Introduction
Species Human
Protein Construction
CD3E/CD3 epsilon [Asp23-Asp126 (C119S, C122S)]
Accession # P07766		 hFc
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD3E/CD3 epsilon hFc Chimera, Human at 2μg/ml (100μl/well) on the plate can bind Biotinylated Anti-CD3 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 36.1 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 35-55 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS，(pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • CD3E/CD3 Epsilon HFc Chimera, Human
    • CD3E/CD3 Epsilon HFc Chimera, Human

    Immobilized CD3E/CD3 epsilon hFc Chimera, Human, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 58.3 ng/ml determined by ELISA.

  • CD3E/CD3 Epsilon HFc Chimera, Human
    • CD3E/CD3 Epsilon HFc Chimera, Human

    CD3E/CD3 epsilon hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E&CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Synonyms CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.