CD3E&CD3G hFc Chimera, Human
CD3E&CD3G HFc Chimera, Human

Immobilized CD3E&CD3G hFc Chimera, Human, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, mFc Tag with the EC50 of 29.8 ng/ml determined by ELISA.

CD3E&CD3G HFc Chimera, Human

The purity of CD3E&CD3G hFc Chimera, Human was greater than 95% as determined by SEC-HPLC.

CD3E&CD3G HFc Chimera, Human

CD3E&CD3G hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD3E&CD3G hFc Chimera, Human

T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E&CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Z03989
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Product Introduction
Species Human
Protein Construction
CD3E & CD3G [Asp23-Asp126 (CD3E) & Gln23-Ser116 (CD3G)]
Accession # P07766 (CD3E) & P09693 (CD3G)		 hFc
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD3E&CD3G hFc Chimera, Human at 1μg/ml (100μl/well) on the plate can bind Anti-CD3E&CD3G Ab.2, mFc Tag . Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 37.8 kDa (CD3E) and 36.5 kDa (CD3G)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS，(pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • CD3E&CD3G HFc Chimera, Human
    • CD3E&CD3G HFc Chimera, Human

    Immobilized CD3E&CD3G hFc Chimera, Human, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, mFc Tag with the EC50 of 29.8 ng/ml determined by ELISA.

  • CD3E&CD3G HFc Chimera, Human
    • CD3E&CD3G HFc Chimera, Human

    The purity of CD3E&CD3G hFc Chimera, Human was greater than 95% as determined by SEC-HPLC.

  • CD3E&CD3G HFc Chimera, Human
    • CD3E&CD3G HFc Chimera, Human

    CD3E&CD3G hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E&CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Synonyms CD3; CD3G; CD3g; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.