EGFR/HER1[Biotin], His & Avi, Human
EGFR/HER1[Biotin], His & Avi, Human

Immobilized EGFR/HER1[Biotin], His & Avi, Human at 1 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA.

EGFR/HER1[Biotin], His & Avi, Human

EGFR/HER1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

EGFR/HER1[Biotin], His & Avi, Human

The purity of EGFR/HER1[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

EGFR/HER1[Biotin], His & Avi, Human

EGFR/HER1[Biotin], His & Avi, Human captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.62 nM as determined in SPR assay (Biacore T200).

EGFR/HER1[Biotin], His & Avi, Human

The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Z03920
¥121,253.00

Ask us a question
Product Introduction
Species Human
Protein Construction
EGFR/HER1 (Leu25-Ser645)
Accession # P00533-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Immobilized EGFR/HER1[Biotin], His & Avi, Human (Cat.No.: Z03920) at 1 μg/ml (100μl/Well) on the streptavidin precoated plate (5μg/ml) can bind Human EGF, hFc Tag
Expression System HEK293
Theoretical Molecular Weight 71.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4 .
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O more than 100 μg/ml.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • EGFR/HER1[Biotin], His & Avi, Human
    • EGFR/HER1[Biotin], His & Avi, Human

    Immobilized EGFR/HER1[Biotin], His & Avi, Human at 1 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA.

  • EGFR/HER1[Biotin], His & Avi, Human
    • EGFR/HER1[Biotin], His & Avi, Human

    EGFR/HER1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • EGFR/HER1[Biotin], His & Avi, Human
    • EGFR/HER1[Biotin], His & Avi, Human

    The purity of EGFR/HER1[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • EGFR/HER1[Biotin], His & Avi, Human
    • EGFR/HER1[Biotin], His & Avi, Human

    EGFR/HER1[Biotin], His & Avi, Human captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.62 nM as determined in SPR assay (Biacore T200).


Background
Target Background The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Synonyms ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.