B7-H3/CD276[Biotin], His & Avi, Human
B7-H3/CD276[Biotin], His & Avi, Human

Immobilized B7-H3/CD276[Biotin], His & Avi, Human at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.4 ng/ml determined by ELISA.

B7-H3/CD276[Biotin], His & Avi, Human

The purity of B7-H3/CD276[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

B7-H3/CD276[Biotin], His & Avi, Human

B7-H3/CD276[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

B7-H3/CD276[Biotin], His & Avi, Human

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers. B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Z03882
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Product Introduction
Species Human
Protein Construction
B7-H3/CD276 (Leu29-Pro245)
Accession # Q5ZPR3-2		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 26.49 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O more than 100 μg/ml.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • B7-H3/CD276[Biotin], His & Avi, Human
    • B7-H3/CD276[Biotin], His & Avi, Human

    Immobilized B7-H3/CD276[Biotin], His & Avi, Human at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.4 ng/ml determined by ELISA.

  • B7-H3/CD276[Biotin], His & Avi, Human
    • B7-H3/CD276[Biotin], His & Avi, Human

    The purity of B7-H3/CD276[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • B7-H3/CD276[Biotin], His & Avi, Human
    • B7-H3/CD276[Biotin], His & Avi, Human

    B7-H3/CD276[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Synonyms B7H3; B7-H3; CD276; PSEC0249; UNQ309; PRO352; B7 homolog 3; CD276

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.