CD3E&CD3D, His, Human
CD3E&CD3D, His, Human

Immobilized CD3E&CD3D, His, Human at 2 μg/ml (100 μl/well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 39.3 ng/ml determined by ELISA.

CD3E&CD3D, His, Human

The purity of CD3E&CD3D, His, Human was greater than 95% as determined by SEC-HPLC.

CD3E&CD3D, His, Human

CD3E&CD3D, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD3E&CD3D, His, Human

Immobilized CD3E&CD3D, His, Human at 2 μg/ml (100 μl/well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 39.3 ng/ml determined by ELISA.

CD3E&CD3D, His, Human

Immobilized CD3E&CD3D, His, Human at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 4.7 ng/ml determined by ELISA.

CD3E&CD3D, His, Human

The T-cell surface glycoproteins CD3E and CD3D, also called CD3 epsilon and CD3 delta chain, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate the T-cell receptor (TCR), TCR signals are transmitted by the CD3 chains CD3D, CD3E, CD3G, and CD3Z. All CD3 chains have immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Z03816
¥89,541.00

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Product Introduction
Species Human
Protein Construction

The protein is composed of CD3E and CD3D

CD3E (Asp23-Asp126)
Accession # P07766		 His tag
CD3D (Phe22-Ala105)
Accession # P04234		 His tag
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Immobilized CD3E&CD3D, His, Human (Cat.No.: Z03816) at 2 μg/ml can bind Anti-CD3 Antibody (OKT3).
Expression System HEK293
Theoretical Molecular Weight 17.9 kDa for CD3E; 16.7 kDa for CD3D
Apparent Molecular Weight Due to glycosylation, the protein migrates to 28-38 kDa and 25 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the lyophilized product remains stable up to 6 months at -20 °C or below as supplied from date of receipt.-80°C for 3 months after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Examples
  • CD3E&CD3D, His, Human
    • CD3E&CD3D, His, Human

    Immobilized CD3E&CD3D, His, Human at 2 μg/ml (100 μl/well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 39.3 ng/ml determined by ELISA.

  • CD3E&CD3D, His, Human
    • CD3E&CD3D, His, Human

    The purity of CD3E&CD3D, His, Human was greater than 95% as determined by SEC-HPLC.

  • CD3E&CD3D, His, Human
    • CD3E&CD3D, His, Human

    CD3E&CD3D, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • CD3E&CD3D, His, Human
    • CD3E&CD3D, His, Human

    Immobilized CD3E&CD3D, His, Human at 2 μg/ml (100 μl/well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 39.3 ng/ml determined by ELISA.

  • CD3E&CD3D, His, Human
    • CD3E&CD3D, His, Human

    Immobilized CD3E&CD3D, His, Human at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 4.7 ng/ml determined by ELISA.


Background
Target Background The T-cell surface glycoproteins CD3E and CD3D, also called CD3 epsilon and CD3 delta chain, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate the T-cell receptor (TCR), TCR signals are transmitted by the CD3 chains CD3D, CD3E, CD3G, and CD3Z. All CD3 chains have immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Synonyms CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.