Activin A, Human
Activin A, Human

Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL.

Activin A, Human

Immobilized Activin A, Human at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 18.6 ng/ml determined by ELISA.

Activin A, Human

Immobilized Activin A, Human at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 2.1 ng/ml determined by ELISA.

Activin A, Human

Immobilized Activin A, Human on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

Activin A, Human

Biotinylated Human Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Activin A, Human, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).

Activin A, Human

Activin-A, a member of the TGF-β superfamily, has fundamental roles in embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation, and tissue fibrosis. It signals through two type I and two type II receptors, activating kinase activity, phosphorylating SMAD2 and 3 intracellular signaling mediators, forming a complex with SMAD4, and translocating to the nucleus to regulate gene expression.
Z03814
¥53,165.00

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Product Introduction
Species Human
Protein Construction
Activin A (Gly311-Ser426)
Accession # P08476
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 0.1EU per μg by the LAL method.
Biological Activity Immobilized Activin A, Human (Cat.No.: Z03814) at 1 μg/ml can bind Human Activin RIIB, His Tag. Activin A, Human (Cat.No.: Z03814) can inhibit proliferation of MPC-11 cells.
Expression System HEK293
Theoretical Molecular Weight 12.97 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in 4 mM HCI.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCI.
Storage & Stability Upon receiving, the lyophilized product remains stable up to 6 months at -20 °C or below as supplied from date of receipt.-80°C for 3 months after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Examples
  • Activin A, Human
    • Activin A, Human

    Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL.

  • Activin A, Human
    • Activin A, Human

    Immobilized Activin A, Human at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 18.6 ng/ml determined by ELISA.

  • Activin A, Human
    • Activin A, Human

    Immobilized Activin A, Human at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 2.1 ng/ml determined by ELISA.

  • Activin A, Human
    • Activin A, Human

    Immobilized Activin A, Human on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

  • Activin A, Human
    • Activin A, Human

    Biotinylated Human Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Activin A, Human, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).


Background
Target Background Activin-A, a member of the TGF-β superfamily, has fundamental roles in embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation, and tissue fibrosis. It signals through two type I and two type II receptors, activating kinase activity, phosphorylating SMAD2 and 3 intracellular signaling mediators, forming a complex with SMAD4, and translocating to the nucleus to regulate gene expression.
Synonyms INHBA; Activin A;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.