IGF-I, Porcine
IGF-I, Porcine

The ED50 as determined by a cell proliferation assay using MCF-7 cells is 1.976 ng/ml

IGF-I, Porcine

Lane 1: 2 µg of IGF-I, Porcine reducing (R)

Lane 2: 2 µg of IGF-I, Porcine reducing (N)

The purity is greater than 95% as analyzed by SDS-PAGE(R)

IGF-I, Porcine

Insulin-like growth factor 1 (IGF-1) is a hormone similar in molecular structure to insulin but has a much higher growth-promoting activity. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. It is able to stimulate glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. It may also play a role in synapse maturation.
Z03790
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Product Introduction
Species Porcine
Protein Construction
IGF-I (Gly49-Ala118)
Accession # P16545
Purity ≥ 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 10.0 ng/ml, measured by a cell proliferation assay using MCF-7 cells.
Expression System E.coli
Theoretical Molecular Weight 7.7 kDa
Apparent Molecular Weight ~ 7.7 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH7.4
Reconstitution Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml
Storage & Stability Upon receiving, this product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles.

Examples
  • IGF-I, Porcine
    • IGF-I, Porcine

    The ED50 as determined by a cell proliferation assay using MCF-7 cells is 1.976 ng/ml

  • IGF-I, Porcine
    • IGF-I, Porcine

    Lane 1: 2 µg of IGF-I, Porcine reducing (R)

    Lane 2: 2 µg of IGF-I, Porcine reducing (N)

    The purity is greater than 95% as analyzed by SDS-PAGE(R)


Background
Target Background Insulin-like growth factor 1 (IGF-1) is a hormone similar in molecular structure to insulin but has a much higher growth-promoting activity. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. It is able to stimulate glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. It may also play a role in synapse maturation.
Synonyms IGF1; Insulin-like growth factor I; IGF-1

For research use only. Not intended for human and animal therapeutic or diagnostic use.