Heat Stable FGF-basic, Human
Heat Stable FGF-basic, Human

The ED50 as determined by a cell proliferation assay using 3T3 cells is 0.04 ng/ml.

Heat Stable FGF-basic, Human

Lane 1: 2 µg of Heat Stable FGF-basic, Human, reducing (R)
The purity is greater than 95% as analyzed by SDS-PAGE

Heat Stable FGF-basic, Human

Heat Stable FGF-basic, Human is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomyocytes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.
Z03754
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Product Introduction
Species Human
Protein Construction

Expressed with an N-terminal Met and several site mutations

Human Heat Stable FGF-basic (Ala135-Ser288)
Accession # P09038
Purity ≥ 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.25 ng/ml as determined by a dose-response proliferation assay using 3T3 cells. Based on the ED50, the calculated specific activity is approximately > 4.0 × 106 units/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.
Expression System E.coli
Theoretical Molecular Weight 17.1 kDa
Apparent Molecular Weight ~17.1 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in PBS up to 100 μg/ml
Storage & Stability Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 °C.

Examples
  • Heat Stable FGF-basic, Human
    • Heat Stable FGF-basic, Human

    The ED50 as determined by a cell proliferation assay using 3T3 cells is 0.04 ng/ml.

  • Heat Stable FGF-basic, Human
    • Heat Stable FGF-basic, Human

    Lane 1: 2 µg of Heat Stable FGF-basic, Human, reducing (R)
    The purity is greater than 95% as analyzed by SDS-PAGE


Background
Target Background Heat Stable FGF-basic, Human is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomyocytes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.
Synonyms Heat Stable FGF-2

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.