PDGF-BB, Bovine
PDGF-BB, Bovine

The ED50 as determined by a cell proliferation assay using mice Balb/c 3T3 cells is 9.86 ng/ml.

PDGF-BB, Bovine

Lane 1: 2 µg of PDGF-BB, Bovine, reducing (R)

The purity is greater than 95% as analyzed by SDS-PAGE

PDGF-BB, Bovine

Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in an animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, and PDGF-AB. It is involved in many biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).
Z03725
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Product Introduction
Species Bovine
Protein Construction
PDGF-BB (Ser82-Thr190)
Accession # B1H0W5
Purity ≥ 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.1 EU/μg of protein by gel clotting method
Biological Activity ED50 < 20.00 ng/ml as determined by a dose-response proliferation assay using murine Balb/c 3T3 cells. Based on the ED50, the calculated specific activity is approximately > 0.5 × 105 IU/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.
Expression System P. pastoris
Theoretical Molecular Weight 12 kDa
Apparent Molecular Weight 10-18 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in 20 mM NaAc, pH 5.0.
Reconstitution Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml.
Storage & Stability Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is storage at -20 °C.

Examples
  • PDGF-BB, Bovine
    • PDGF-BB, Bovine

    The ED50 as determined by a cell proliferation assay using mice Balb/c 3T3 cells is 9.86 ng/ml.

  • PDGF-BB, Bovine
    • PDGF-BB, Bovine

    Lane 1: 2 µg of PDGF-BB, Bovine, reducing (R)

    The purity is greater than 95% as analyzed by SDS-PAGE


Background
Target Background Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in an animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, and PDGF-AB. It is involved in many biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).
Synonyms PDGFBB; PDGF-BB

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.