IGF-I, Bovine
IGF-I, Bovine

ED50 < 5 ng/ml, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0× 105 units/mg.

IGF-I, Bovine

Insulin-like growth factor 1 (IGF-1), also called Somatomedin, is a hormone similar in molecular structure to insulin but has a much higher growth-promoting activity. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. It is able to stimulate glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. It may also play a role in synapse maturation.
Z03688
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Product Introduction
Species Bovine
Protein Construction

Expressed with an N-terminal Met.

IGF-I (Gly50-Ala119)
Accession # P07455
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 15.0 ng/ml, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 6.7 × 104 units/mg.
Expression System E. coli
Apparent Molecular Weight ~7.7 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • IGF-I, Bovine
    • IGF-I, Bovine

    ED50 < 5 ng/ml, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0× 105 units/mg.


Background
Target Background Insulin-like growth factor 1 (IGF-1), also called Somatomedin, is a hormone similar in molecular structure to insulin but has a much higher growth-promoting activity. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. It is able to stimulate glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. It may also play a role in synapse maturation.
Synonyms Somatomedin; IGF1; Insulin-like growth factor I; IGF-1

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.