CD40, His, Human
CD40, His, Human

Immobilized Human CD40, His Tag at 0.5 μg/ml (100 μl/Well). Dose response curve for Human CD40L, hFc Tag with the EC50 of 0.22 μg/ml determined by ELISA.

CD40, His, Human

Human CD40 on SDS-PAGE under reducing conditions. The purity is greater than 95%.

CD40, His, Human

The purity of Human CD40 is greater than 95% as determined by SEC-HPLC.

CD40, His, Human

CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Z03647
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Product Introduction
Species Human
Protein Construction
CD40 (Glu21-Arg193)
Accession # P25942		 Poly-His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level ≤ 1 EU/μg of protein by LAL method
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD40, His, Human at 2μg/ml (100μl/Well) on the plate can bind Human CD40 Ligand (Trimer) , hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 20 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O more than 100 μg/ml.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • CD40, His, Human
    • CD40, His, Human

    Immobilized Human CD40, His Tag at 0.5 μg/ml (100 μl/Well). Dose response curve for Human CD40L, hFc Tag with the EC50 of 0.22 μg/ml determined by ELISA.

  • CD40, His, Human
    • CD40, His, Human

    Human CD40 on SDS-PAGE under reducing conditions. The purity is greater than 95%.

  • CD40, His, Human
    • CD40, His, Human

    The purity of Human CD40 is greater than 95% as determined by SEC-HPLC.


Background
Target Background CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Synonyms CD40; CD40L receptor; TNFRSF5; B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.