EpCAM/Trop1, His, Human
EpCAM/Trop1, His, Human

Immobilized Human EpCAM at 0.2 μg/ml(100 μl/Well). Dose response curve for Anti-Trop1 Ab with the EC50 of 8.7 ng/ml determined by ELISA.

EpCAM/Trop1, His, Human

Recombinant EpCAM on SDS-PAGE under reducing conditions, the purity is greater than 95%.

EpCAM/Trop1, His, Human

The purity of EpCAM is greater than 95% as determined by SEC-HPLC.

EpCAM/Trop1, His, Human

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Z03635
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Product Introduction
Species Human
Protein Construction
EpCAM/Trop1 (Gln24-Lys245)
Accession # P16422		 Poly-His
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level ≤ 1 EU/μg of protein by LAL method
Biological Activity Immobilized Human EpCAM at 0.2 μg/ml (100 μl/Well). Dose response curve for  Anti-Trop1 Ab with the EC50 of 8.7 ng/ml determined by ELISA.
Expression System Expi293
Apparent Molecular Weight 30~45 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4. Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Avoid repeated freeze-thaw cycles.

Examples
  • EpCAM/Trop1, His, Human
    • EpCAM/Trop1, His, Human

    Immobilized Human EpCAM at 0.2 μg/ml(100 μl/Well). Dose response curve for Anti-Trop1 Ab with the EC50 of 8.7 ng/ml determined by ELISA.

  • EpCAM/Trop1, His, Human
    • EpCAM/Trop1, His, Human

    Recombinant EpCAM on SDS-PAGE under reducing conditions, the purity is greater than 95%.

  • EpCAM/Trop1, His, Human
    • EpCAM/Trop1, His, Human

    The purity of EpCAM is greater than 95% as determined by SEC-HPLC.


Background
Target Background Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Synonyms CD326 antigen; CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; ACSTD1; antigen identified by monoclonal AUA1; Cell surface glycoprotein Trop-1; DIAR5; Epithelial glycoprotein 314; Epithelial glycoprotein; gp40; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MOC31; TACST-1; TACSTD1; TROP1; Tumor-associated calcium signal transducer 1CO-17A

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.