EpCAM/Trop1, His, Human
EpCAM/Trop1, His, Human

Immobilized Human EpCAM at 0.2 μg/ml(100 μl/Well). Dose response curve for Anti-Trop1 Ab with the EC50 of 8.7 ng/ml determined by ELISA.

EpCAM/Trop1, His, Human

Recombinant EpCAM on SDS-PAGE under reducing conditions, the purity is greater than 95%.

EpCAM/Trop1, His, Human

The purity of EpCAM is greater than 95% as determined by SEC-HPLC.

EpCAM/Trop1, His, Human

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Z03635
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Product Introduction
Species Human
Protein Construction
EpCAM/Trop1 (Gln24-Lys245)
Accession # P16422		 Poly-His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level ≤ 1 EU/μg of protein by LAL method
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized EpCAM/Trop1, His, Human at 0.2μg/ml (100μl/well) on the plate can bind Anti­EpCAM Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 28.2 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O more than 100 μg/ml.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • EpCAM/Trop1, His, Human
    • EpCAM/Trop1, His, Human

    Immobilized Human EpCAM at 0.2 μg/ml(100 μl/Well). Dose response curve for Anti-Trop1 Ab with the EC50 of 8.7 ng/ml determined by ELISA.

  • EpCAM/Trop1, His, Human
    • EpCAM/Trop1, His, Human

    Recombinant EpCAM on SDS-PAGE under reducing conditions, the purity is greater than 95%.

  • EpCAM/Trop1, His, Human
    • EpCAM/Trop1, His, Human

    The purity of EpCAM is greater than 95% as determined by SEC-HPLC.


Background
Target Background Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Synonyms CD326 antigen; CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; ACSTD1; antigen identified by monoclonal AUA1; Cell surface glycoprotein Trop-1; DIAR5; Epithelial glycoprotein 314; Epithelial glycoprotein; gp40; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MOC31; TACST-1; TACSTD1; TROP1; Tumor-associated calcium signal transducer 1CO-17A

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.