CD73, His, Human
CD73, His, Human

The purity of Human CD73 is greater than 95% as determined by SEC-HPLC.

CD73, His, Human

Immobilized Human CD73, His Tag at 0.5 μg/ml (100 μl/well). Dose response curve for Anti-CD73 Ab with the EC50 of 16.9 ng/ml determined by ELISA.

CD73, His, Human

Human CD73 on SDS-PAGE under reducing conditions. The purity is greater than 95%.

CD73, His, Human

CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A  deficiency of CD73 occurs in a variety of immunodeficiency diseases.
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Product Introduction
Species Human
Protein Construction
CD73 (Trp27-Lys547)
Accession # P21589		 Poly-His
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level ≤ 1 EU/μg of protein by LAL method
Biological Activity Immobilized Human CD73, His at 0.5 μg/ml (100 μl/Well). Dose response curve for Anti-CD73 Ab with the EC50 of 19.3 ng/ml determined by ELISA.
Expression System Expi293
Apparent Molecular Weight ~58.8 kDa, on SDS-PAGE under reducing conditions.
Formulation Supplied as a 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl, 4 mM CaCl2, 20% glycerol, pH 7.5.
Concentration Please refer to the COA for the specific lot.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Avoid repeated freeze-thaw cycles.

Examples
  • CD73, His, Human
    • CD73, His, Human

    The purity of Human CD73 is greater than 95% as determined by SEC-HPLC.

  • CD73, His, Human
    • CD73, His, Human

    Immobilized Human CD73, His Tag at 0.5 μg/ml (100 μl/well). Dose response curve for Anti-CD73 Ab with the EC50 of 16.9 ng/ml determined by ELISA.

  • CD73, His, Human
    • CD73, His, Human

    Human CD73 on SDS-PAGE under reducing conditions. The purity is greater than 95%.


Background
Target Background CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A  deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Synonyms CD73 antigen; CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73);  E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; Purine 5-Prime-Nucleotidase

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.