Siglec-15, hFc, Human
Siglec-15 Fc Chimera, Human

Immobilized Anti Siglec15 at 1 μg/ml (100 μl/well) can bind Siglec15 Fc Chimera, Human with a EC50 = 5.184 ng/ml and a linear range of 0.064−200 ng/ml when detected by Mouse Anti–Human IgG Fc-HRP. Background was subtracted from data points before curve fitting.

Siglec-15 Fc Chimera, Human

Siglec-15 preferentially recognizes the Neu5Acalpha2-6GalNAcalpha- structure. Siglec-15 associates with the activating adaptor proteins DNAX activation protein (DAP)12 and DAP10 via its lysine residue in the transmembrane domain. Siglec-15 is the second human Siglec identified to have an activating signaling potential; unlike Siglec-14, however, it does not have an inhibitory counterpart.
Z03571
¥28,819.00

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Product Introduction
Species Human
Protein Construction
Siglec-15 (Phe20-Thr263)
Accession # Q6ZMC9		 hFc
N-term C-term
Purity > 90% as analyzed by SDS-PAGE
Endotoxin Level < 1 EU/μg of protein by gel clotting method
Biological Activity Immobilized Anti-Siglec15 at 1.0 μg/ml (100 μl/well) can bind Siglec-15, hFc, Human with a EC50 = 5.184 ng/ml and a linear range of 0.064−200.0 ng/ml when detected by Mouse Anti–Human IgG Fc-HRP.
Expression System HEK 293
Apparent Molecular Weight ~54.3 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • Siglec-15 Fc Chimera, Human
    • Siglec-15 Fc Chimera, Human

    Immobilized Anti Siglec15 at 1 μg/ml (100 μl/well) can bind Siglec15 Fc Chimera, Human with a EC50 = 5.184 ng/ml and a linear range of 0.064−200 ng/ml when detected by Mouse Anti–Human IgG Fc-HRP. Background was subtracted from data points before curve fitting.


Background
Target Background Siglec-15 preferentially recognizes the Neu5Acalpha2-6GalNAcalpha- structure. Siglec-15 associates with the activating adaptor proteins DNAX activation protein (DAP)12 and DAP10 via its lysine residue in the transmembrane domain. Siglec-15 is the second human Siglec identified to have an activating signaling potential; unlike Siglec-14, however, it does not have an inhibitory counterpart.
Synonyms CD33L3; HsT1361; SIGLEC-15

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.