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SARS-CoV-2 Spike Protein (S1)

Western blot analysis of SARS-CoV-2 Spike protein (S1) (GenScript, Z03501) using S1 Antibody(GenScript, A02053).
Lane 1: 10 ng of Z03501
Lane 2: 5ng of Z03501

SARS-CoV-2 Spike Protein (S1)

Immobilized SARS-CoV-2 Spike protein (S1) (Cat. No. Z03501) at 1 μg/mL can bind ACE-2 Fc Chimera, Human (Cat. No. Z03484) with a serial dilution.
Mouse Anti-Human IgG Fc Antibody[HRP], mAb (Cat.No. A01854) is used as a secondary antibody (0.1 μg/mL).

SARS-CoV-2 Spike Protein (S1)

Lane 1: 2μg of SARS-CoV-2 Spike protein (S1), reducing(R)
Lane 2: 2μg of SARS-CoV-2 Spike protein (S1), non-reducing(NR)
> 85% as analyzed by SDS-PAGE

SARS-CoV-2 Spike protein (S1)

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. S protein plays an important role in the induction of neutralizing-antibodies and T-cell responses, as well as protective immunity.
Z03501
¥68,875.00

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Product Introduction
Species SARS-CoV-2
Protein Construction
S1 protein (Gln14-Arg685)
Accession # P0DTC2
Purity > 90% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity SARS-CoV-2 Spike protein (S1) can bind with Human ACE2 in functional ELISA assay.
Expression System 293 Cells
Theoretical Molecular Weight 78.3 kDa
(Additional amino acids from the vector are included. The sequence of them is confidential.)
Formulation Supplied as a solution in PBS pH 7.4
Concentration Please refer to the COA for the specific lot.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at -20°C or below. Avoid repeated freeze-thaw cycles.

Examples
  • SARS-CoV-2 Spike Protein (S1)
    • SARS-CoV-2 Spike Protein (S1)

    Western blot analysis of SARS-CoV-2 Spike protein (S1) (GenScript, Z03501) using S1 Antibody(GenScript, A02053).
    Lane 1: 10 ng of Z03501
    Lane 2: 5ng of Z03501

  • SARS-CoV-2 Spike Protein (S1)
    • SARS-CoV-2 Spike Protein (S1)

    Immobilized SARS-CoV-2 Spike protein (S1) (Cat. No. Z03501) at 1 μg/mL can bind ACE-2 Fc Chimera, Human (Cat. No. Z03484) with a serial dilution.
    Mouse Anti-Human IgG Fc Antibody[HRP], mAb (Cat.No. A01854) is used as a secondary antibody (0.1 μg/mL).

  • SARS-CoV-2 Spike Protein (S1)
    • SARS-CoV-2 Spike Protein (S1)

    Lane 1: 2μg of SARS-CoV-2 Spike protein (S1), reducing(R)
    Lane 2: 2μg of SARS-CoV-2 Spike protein (S1), non-reducing(NR)
    > 85% as analyzed by SDS-PAGE


Background
Target Background SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. S protein plays an important role in the induction of neutralizing-antibodies and T-cell responses, as well as protective immunity.
Synonyms SARS-CoV-2 S1 protein; 2019-nCoV S1 protein

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.