PD-L1, hFc, Mouse
PD-L1 Fc Chimera, Mouse

Immobilized PD-L1 Fc Chimera,Mouse at 2 μg/ml (100 μl/well) can bind PD-1 protein, Mouse, Recombinant (ECD,His Tag) with EC50=17.578 µg/ml when detected by His-HRP. Background was subtracted from data points before curve fitting.

PD-L1 Fc Chimera, Mouse

Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a protein that in humans is encoded by the CD274 gene. PD-L1 is a 40 kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2. PD-L1 binds to its receptor, PD-1, found on activated T cells, B cells, and myeloid cells, to modulate activation or inhibition.
Z03384
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Product Introduction
Species Mouse
Protein Construction
PD-L1 (Phe19-Thr238)
Accession # Q9EP73		 hFc
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 1 EU/μg of protein by gel clotting method
Biological Activity Immobilized PD-L1, hFc, Mouse at 2.0 μg/ml (100 μl/well) can bind PD-1 protein, Mouse, Recombinant (ECD,His Tag) with EC50=17.578 µg/ml when detected by His-HRP.
Expression System HEK 293
Apparent Molecular Weight ~70.9KDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • PD-L1 Fc Chimera, Mouse
    • PD-L1 Fc Chimera, Mouse

    Immobilized PD-L1 Fc Chimera,Mouse at 2 μg/ml (100 μl/well) can bind PD-1 protein, Mouse, Recombinant (ECD,His Tag) with EC50=17.578 µg/ml when detected by His-HRP. Background was subtracted from data points before curve fitting.


Background
Target Background Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a protein that in humans is encoded by the CD274 gene. PD-L1 is a 40 kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2. PD-L1 binds to its receptor, PD-1, found on activated T cells, B cells, and myeloid cells, to modulate activation or inhibition.
Synonyms CD274; B7-H; B7H1; PDCD1L1; PDCD1LG1; PDL1; CD274 molecule; Programmed cell death ligand 1; hPD-L1

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.