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Heregulin β-1, Human

ED50 ≤ 0.5 ng/ml, determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells.

Heregulin β-1, Human

Lane 1: 2 μg of Heregulin β-1, Human, non-reducing (NR)
Lane 2: 2 μg of Heregulin β-1, Human, reducing (R)
> 95% as analyzed by SDS-PAGE

Heregulin β-1, Human

Neuregulins or neuroregulins are a family of four structurally related proteins (NRG1, NRG2, NRG3 and NRG4) that are members of the EGF family of proteins. Studies indicate neuregulins function in nervous system development with essential roles in vertebrate embryogenesis including: cardiac development, Schwann cell and oligodendrocyte differentiation, certain aspects of neuronal development, and the formation of neuromuscular synapses. Neuregulin 1 is essential for the normal development of the nervous system and the heart. It is produced in numerous isoforms by alternative splicing, allowing it to perform a variety of functions. All NRG1 isoforms contain an EGF-like domain that is required for direct binding to ErbB3 or ErbB4 receptor tyrosine kinases. The transmembrane NRG1 isoforms contain an extracellular domain that can be proteolytically cleaved to release soluble growth factors.
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Product Introduction
Species Human
Protein Construction
Heregulin β-1 (Thr176-Lys246)
Accession # Q02297-6
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 ≤ 0.5 ng/ml, determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells.
Expression System CHO
Apparent Molecular Weight ~7.5 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • Heregulin β-1, Human
    • Heregulin β-1, Human

    ED50 ≤ 0.5 ng/ml, determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells.

  • Heregulin β-1, Human
    • Heregulin β-1, Human

    Lane 1: 2 μg of Heregulin β-1, Human, non-reducing (NR)
    Lane 2: 2 μg of Heregulin β-1, Human, reducing (R)
    > 95% as analyzed by SDS-PAGE


Background
Target Background Neuregulins or neuroregulins are a family of four structurally related proteins (NRG1, NRG2, NRG3 and NRG4) that are members of the EGF family of proteins. Studies indicate neuregulins function in nervous system development with essential roles in vertebrate embryogenesis including: cardiac development, Schwann cell and oligodendrocyte differentiation, certain aspects of neuronal development, and the formation of neuromuscular synapses. Neuregulin 1 is essential for the normal development of the nervous system and the heart. It is produced in numerous isoforms by alternative splicing, allowing it to perform a variety of functions. All NRG1 isoforms contain an EGF-like domain that is required for direct binding to ErbB3 or ErbB4 receptor tyrosine kinases. The transmembrane NRG1 isoforms contain an extracellular domain that can be proteolytically cleaved to release soluble growth factors.
Synonyms Heregulin beta-1; NRG1-beta 1; neuregulin

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.