リコンビナントタンパク質＿精製したリコンビナントタンパク質 » リコンビナントサイトカイン-免疫活性タンパク質 » IL-12, Mouse
IL-12, Mouse

Lane 1: 2 μg of IL-12, Mouse, non-reducing (NR)
Lane 2: 2 μg of IL-12, Mouse, reducing (R)
> 95% as analyzed by SDS-PAGE

IL-12, Mouse

Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals throughtheIL-12 receptor complex, which is comprised of IL-12 Rβ1 and IL-12 Rβ2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2.
Z03211
¥31,356.00

Ask us a question
Product Introduction
Species Mouse
Protein Construction
IL-12 p35 (Arg23-Ser215)
Accession # P43431
IL-12 p40 (Met23-Ser335)
Accession # P43432
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.15 ng/ml, measured in a cell proliferation assay using 2D6 cells.
Expression System CHO
Apparent Molecular Weight 27~29 kDa (p35), 45~50 kDa (p40), on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • IL-12, Mouse
    • IL-12, Mouse

    Lane 1: 2 μg of IL-12, Mouse, non-reducing (NR)
    Lane 2: 2 μg of IL-12, Mouse, reducing (R)
    > 95% as analyzed by SDS-PAGE


Background
Target Background Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals throughtheIL-12 receptor complex, which is comprised of IL-12 Rβ1 and IL-12 Rβ2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2.
Synonyms IL12; NKSF; TCMF; CLMF; TSF; CTL maturation factor; Cytotoxic lymphocyte maturation factor

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.