リコンビナントタンパク質＿精製したリコンビナントタンパク質 » リコンビナント成長因子 » PDGF-BB, Rat
PDGF-BB, Rat

Lane 1: 2 μg of PDGF-BB, Rat, non-reducing (NR)
Lane 2: 2 μg of PDGF-BB, Rat, reducing (R)
> 95% as analyzed by SDS-PAGE

PDGF-BB, Rat

Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.
Z03179
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Product Introduction
Species Rat
Protein Construction

Expressed with an N-terminal Met.

PDGF-BB (Ser74-Thr182)
Accession # Q05028
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 2.0 ng/ml, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 5.0 × 105 units/mg.
Expression System E. coli
Apparent Molecular Weight ~24.6 kDa, on SDS-PAGE under non-reducing conditions.
Formulation Lyophilized after extensive dialysis against 20 mM acetic acid.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in 20 mM acetic acid up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • PDGF-BB, Rat
    • PDGF-BB, Rat

    Lane 1: 2 μg of PDGF-BB, Rat, non-reducing (NR)
    Lane 2: 2 μg of PDGF-BB, Rat, reducing (R)
    > 95% as analyzed by SDS-PAGE


Background
Target Background Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.
Synonyms PDGFBB; IBGC5; PDGF-2; PDGF2; SIS; SSV; c-sis; platelet derived growth factor subunit B

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.