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G-CSF, Mouse

ED50 < 0.02 ng/ml, measured in a cell proliferation assay using NFS-60 cells.

G-CSF, Mouse

Lane 1: 3 μg of G-CSF, Mouse, non-reducing (NR)
Lane 2: 3 μg of G-CSF, Mouse, reducing (R)
> 95% as analyzed by SDS-PAGE

G-CSF, Mouse

Granulocyte Colony-Stimulating Factor (G-CSF), also known as CSF-3 and MGI-1G, is a cytokine and hormone belonging to the IL-6 superfamily. It is expressed by monocytes, macrophages, endothelial cells, fibroblasts and bone marrow stroma. G-CSF stimulates the bone marrow to produce granulocytes and stem cells, and specifically stimulates the proliferation and differentiation of the neutrophilic granulocyte lineage. G-CSF has been used to stimulate white blood cell production after chemotherapy. It has also been used to boost the number of hematopoietic stem cells after bone marrow transplantation.
Z03163
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Product Introduction
Species Mouse
Protein Construction
G-CSF (Val31-Ala208)
Accession # P09920
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.02 ng/ml, measured in a cell proliferation assay using NFS-60 cells.
Expression System CHO
Apparent Molecular Weight 22~24 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • G-CSF, Mouse
    • G-CSF, Mouse

    ED50 < 0.02 ng/ml, measured in a cell proliferation assay using NFS-60 cells.

  • G-CSF, Mouse
    • G-CSF, Mouse

    Lane 1: 3 μg of G-CSF, Mouse, non-reducing (NR)
    Lane 2: 3 μg of G-CSF, Mouse, reducing (R)
    > 95% as analyzed by SDS-PAGE


Background
Target Background Granulocyte Colony-Stimulating Factor (G-CSF), also known as CSF-3 and MGI-1G, is a cytokine and hormone belonging to the IL-6 superfamily. It is expressed by monocytes, macrophages, endothelial cells, fibroblasts and bone marrow stroma. G-CSF stimulates the bone marrow to produce granulocytes and stem cells, and specifically stimulates the proliferation and differentiation of the neutrophilic granulocyte lineage. G-CSF has been used to stimulate white blood cell production after chemotherapy. It has also been used to boost the number of hematopoietic stem cells after bone marrow transplantation.
Synonyms CSF3; C17orf33; CSF3OS; GCSF; colony stimulating factor 3; CSF-3; Granulocyte Colony-Stimulating Factor

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.