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KGF/FGF-7, Mouse

ED50 < 2.0 ng/ml, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0 × 105 units/mg.

KGF/FGF-7, Mouse

Lane 1: 2 μg of KGF/FGF-7, Mouse, reducing (R)
Lane 2: 2 μg of KGF/FGF-7, Mouse, non-reducing (NR)
> 95% as analyzed by SDS-PAGE

KGF/FGF-7, Mouse

Keratinocyte Growth Factor (KGF) is a highly specific epithelial mitogen produced by fibroblasts and mesenchymal stem cells. KGF belongs to the heparin binding Fibroblast Growth Factor (FGF) family, and is known as FGF-7. However, in contrast to FGF-1, which binds to all known FGF receptors with high affinity, KGF only binds to a splice variant of the FGF receptor, FGFR2-IIIb. FGFR2-IIIb is expressedby most epithelial cells, indicating KGF’s roleas a paracrine mediator. KGF induces the differentiation and proliferation of various epithelial cells such as keratinocytes in the epidermis, hair follicles and sebaceous glands., KGF is also responsible for wound repair of various tissuesincluding lung, bladder, and kidney.
Z03154
¥18,125.00

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Product Introduction
Species Mouse
Protein Construction
FGF-7 (Cys32-Thr194)
Accession # P36363
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 2.0 ng/ml, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0 × 105 units/mg.
Expression System E. coli
Apparent Molecular Weight ~18.7 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • KGF/FGF-7, Mouse
    • KGF/FGF-7, Mouse

    ED50 < 2.0 ng/ml, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0 × 105 units/mg.

  • KGF/FGF-7, Mouse
    • KGF/FGF-7, Mouse

    Lane 1: 2 μg of KGF/FGF-7, Mouse, reducing (R)
    Lane 2: 2 μg of KGF/FGF-7, Mouse, non-reducing (NR)
    > 95% as analyzed by SDS-PAGE


Background
Target Background Keratinocyte Growth Factor (KGF) is a highly specific epithelial mitogen produced by fibroblasts and mesenchymal stem cells. KGF belongs to the heparin binding Fibroblast Growth Factor (FGF) family, and is known as FGF-7. However, in contrast to FGF-1, which binds to all known FGF receptors with high affinity, KGF only binds to a splice variant of the FGF receptor, FGFR2-IIIb. FGFR2-IIIb is expressedby most epithelial cells, indicating KGF’s roleas a paracrine mediator. KGF induces the differentiation and proliferation of various epithelial cells such as keratinocytes in the epidermis, hair follicles and sebaceous glands., KGF is also responsible for wound repair of various tissuesincluding lung, bladder, and kidney.
Synonyms Keratinocyte Growth Factor; Fibroblast Growth Factor-7; HBGF-7; FGF7

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.