リコンビナントタンパク質＿精製したリコンビナントタンパク質 » リコンビナントサイトカイン-免疫活性タンパク質 » IL-11, Mouse (HEK 293-expressed)
IL-11, Mouse(HEK 293-expressed)

Lane 1: 2 μg of IL-11, Mouse(HEK 293-expressed), reducing (R)
Lane 2: 2 μg of IL-11, Mouse(HEK 293-expressed), non-reducing (NR)
> 95% as analyzed by SDS-PAGE

IL-11, Mouse(HEK 293-expressed)

Interleukin-11 (IL-11) is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive mouse plasmacytoma cell line T11. IL-11 contains no cysteine residues or potential glycosylation sites. IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cell.
Z03052
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Product Introduction
Species Mouse
Protein Construction
IL-11 (Gly23-Leu199)
Accession # P47873
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 3.0 ng/ml, measured in a cell proliferation assay using TF-1 cells.
Expression System HEK 293
Apparent Molecular Weight ~21 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • IL-11, Mouse(HEK 293-expressed)
    • IL-11, Mouse(HEK 293-expressed)

    Lane 1: 2 μg of IL-11, Mouse(HEK 293-expressed), reducing (R)
    Lane 2: 2 μg of IL-11, Mouse(HEK 293-expressed), non-reducing (NR)
    > 95% as analyzed by SDS-PAGE


Background
Target Background Interleukin-11 (IL-11) is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive mouse plasmacytoma cell line T11. IL-11 contains no cysteine residues or potential glycosylation sites. IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cell.
Synonyms IL11; AGIF; IL-11; interleukin 11

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.