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IL-6, Human

Biological Activity
IL-6, Human (Cat. No. Z03034) stimulates cell proliferation of 7TD1 cells. The ED50 for this effect is less than 0.1ng/mL.

IL-6, Human

2 μg of IL-6, Human (Cat. No. Z03034) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining

IL-6, Human

Interleukin-6 (IL-6), also known as BSF-2, CDF and IFNB2, is a pleiotropic cytokine that participates in both pro-inflammatory and anti-inflammatory responses. It is produced mainly by T cells, macrophages, monocytes, endothelial cells and muscle cells. IL-6 binds to IL-6 receptor (IL-6R) to trigger the association of IL-6R with gp130, inducing signal transduction through JAKs and STATs. The biological functions of IL-6 are diverse. It stimulates B cell differentiation and antibody production, myeloma and plasmacytoma growth, and nerve cell differentiation. It also acts as a myokine, produced by muscle cells in response to muscle contraction and released into the blood stream to help break down fats and improve insulin resistance.
Z03034
¥14,319.00

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Product Introduction
Species Human
Protein Construction

Expressed with an N-terminal Met.

IL-6 (Pro29-Met212)
Accession # Q75MH2
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 1.0 ng/ml, measured in a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1.0 × 106 units/mg.
Expression System E. coli
Apparent Molecular Weight ~20.9 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • IL-6, Human
    • IL-6, Human

    Biological Activity
    IL-6, Human (Cat. No. Z03034) stimulates cell proliferation of 7TD1 cells. The ED50 for this effect is less than 0.1ng/mL.

  • IL-6, Human
    • IL-6, Human

    2 μg of IL-6, Human (Cat. No. Z03034) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining


Background
Target Background Interleukin-6 (IL-6), also known as BSF-2, CDF and IFNB2, is a pleiotropic cytokine that participates in both pro-inflammatory and anti-inflammatory responses. It is produced mainly by T cells, macrophages, monocytes, endothelial cells and muscle cells. IL-6 binds to IL-6 receptor (IL-6R) to trigger the association of IL-6R with gp130, inducing signal transduction through JAKs and STATs. The biological functions of IL-6 are diverse. It stimulates B cell differentiation and antibody production, myeloma and plasmacytoma growth, and nerve cell differentiation. It also acts as a myokine, produced by muscle cells in response to muscle contraction and released into the blood stream to help break down fats and improve insulin resistance.
Synonyms IL6; 26 kDa protein; IFN-β2; IFN-b2; B cell differentiation factor; BCDF; BSF-2; HPGF; HSF; MGI-2

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.