リコンビナントタンパク質＿精製したリコンビナントタンパク質 » リコンビナントサイトカイン-免疫活性タンパク質 » IFN-α 2b, Human
IFN-α 2b, Human

Biological Activity
IFN-alpha 2b, Human (Cat. No. Z03002) induced cytotoxicity of R&D TF-1 cells. The ED50 for this effect is typically 20-50pg/mL.

IFN-α 2b, Human

2 μg of IFN-α 2b, Human (Cat. No. Z03002) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.2 μg of IFN-α 2b, Human (Cat. No. Z03002) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

IFN-α 2b, Human

Interferon-Alpha 2b (IFN-Alpha 2b) produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a,b,c) signaling through IFNAR2. IFN-alpha 2b shares 99.4% aa sequence identity with both IFN-alpha 2a and 2c. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.
Z03002
¥7,250.00

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Product Introduction
Species Human
Protein Construction
IFN-α 2b [Cys24-Glu188 (Lys46Arg)]
Accession # P01563
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.05 ng/ml, measured by a cytotoxicity assay using TF-1 Cells, corresponding to a specific activity of > 2.0 × 107 units/mg.
Expression System E. coli
Apparent Molecular Weight ~19.2 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • IFN-α 2b, Human
    • IFN-α 2b, Human

    Biological Activity
    IFN-alpha 2b, Human (Cat. No. Z03002) induced cytotoxicity of R&D TF-1 cells. The ED50 for this effect is typically 20-50pg/mL.

  • IFN-α 2b, Human
    • IFN-α 2b, Human

    2 μg of IFN-α 2b, Human (Cat. No. Z03002) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.2 μg of IFN-α 2b, Human (Cat. No. Z03002) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.


Background
Target Background Interferon-Alpha 2b (IFN-Alpha 2b) produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a,b,c) signaling through IFNAR2. IFN-alpha 2b shares 99.4% aa sequence identity with both IFN-alpha 2a and 2c. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.
Synonyms Interferon alpha 2b; IFNA; INFA2; MGC125764; MGC125765

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.