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G-CSF, Human

Biological Activity
G-CSF, Human (Cat. No. Z02974) stimulates cell proliferation of M-NFS-60 cells. The ED50 for this effect is typically 20-100pg/mL.

G-CSF, Human

2 μg of G-CSF, Human (Cat. No. Z02974) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining

G-CSF, Human

Granulocyte Colony-Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17.
Z02974
¥16,131.00

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Product Introduction
Species Human
Protein Construction

Expressed with an N-terminal Met.

G-CSF (Thr27-Pro200)
Accession # Q8N4W3
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.1 ng/ml, measured by a cell proliferation assay of M-NFS-60 cells, corresponding to a specific activity of > 1.0 × 107 IU/mg.
Expression System E. coli
Apparent Molecular Weight ~18.8 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against 25 mM Tris, pH 8.0.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • G-CSF, Human
    • G-CSF, Human

    Biological Activity
    G-CSF, Human (Cat. No. Z02974) stimulates cell proliferation of M-NFS-60 cells. The ED50 for this effect is typically 20-100pg/mL.

  • G-CSF, Human
    • G-CSF, Human

    2 μg of G-CSF, Human (Cat. No. Z02974) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining


Background
Target Background Granulocyte Colony-Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17.
Synonyms CSF3; C17orf33; CSF3OS; GCSF; colony stimulating factor 3; CSF-3; Granulocyte Colony-Stimulating Factor

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.