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FGF-acidic, Human

ED₅₀<0.3ng/ml, measured by a cell proliferation assay of 3T3 Cells, corresponding to a specific activity of >3.3 × 10⁶ IU/mg in the presence of 10 μg/ml of heparin.

FGF-acidic, Human

Fibroblast Growth Factor- acidic (FGF-acidic), also known as FGF-1 and endothelial cell growth factor, is a member of the FGF family which currently contain 23 members. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF acidic has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. As with other FGF's, FGF acidic exhibits considerable species cross reactivity. FGF acidic and FGF basic stimulate the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin.
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Product Introduction
Species Human
Protein Construction
FGF-acidic (Phe16-Asp155)
Accession # P05230-1
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.3 ng/ml, measured by a cell proliferation assay of 3T3 Cells, corresponding to a specific activity of > 3.3 × 106 IU/mg in the presence of 10.0 μg/ml of heparin.
Expression System E. coli
Apparent Molecular Weight ~15.8 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • FGF-acidic, Human
    • FGF-acidic, Human

    ED₅₀<0.3ng/ml, measured by a cell proliferation assay of 3T3 Cells, corresponding to a specific activity of >3.3 × 10⁶ IU/mg in the presence of 10 μg/ml of heparin.


Background
Target Background Fibroblast Growth Factor- acidic (FGF-acidic), also known as FGF-1 and endothelial cell growth factor, is a member of the FGF family which currently contain 23 members. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF acidic has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. As with other FGF's, FGF acidic exhibits considerable species cross reactivity. FGF acidic and FGF basic stimulate the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin.
Synonyms Fibroblast Growth Factor-acidic; HBGF-1; ECGF-beta; FIBP; FGFIBP; FIBP-1; ECGF; ECGFA; GLIO703; FGF1; FGF-a

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.