リコンビナントタンパク質＿精製したリコンビナントタンパク質 » リコンビナントサイトカイン-免疫活性タンパク質 » TNF-α, Mouse (P. pastoris-expressed)
TNF-α, Mouse (P. Pastoris-expressed)

ED50 < 0.01 ng/ml, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of > 1.0 × 108 units/mg.

TNF-α, Mouse (P. Pastoris-expressed)

Lane 1: 2 μg of TNF-α, Mouse (P. pastoris-expressed), reducing (R)
> 95% as analyzed by SDS-PAGE

TNF-α, Mouse (P. pastoris-expressed)

Tumor necrosis factor alpha (TNF-α) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. Mouse TNF-α occurs as a membrane-anchored form. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and mouse TNF-α show approximately 79% homology at the amino acid level and crossreactivity between the two species.
Z02918
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Product Introduction
Species Mouse
Protein Construction
TNF-α (Ser84-Leu235)
Accession # P06804
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 1 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.01 ng/ml, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of > 1.0 × 108 units/mg.
Expression System P. pastoris
Apparent Molecular Weight ~17 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • TNF-α, Mouse (P. Pastoris-expressed)
    • TNF-α, Mouse (P. Pastoris-expressed)

    ED50 < 0.01 ng/ml, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of > 1.0 × 108 units/mg.

  • TNF-α, Mouse (P. Pastoris-expressed)
    • TNF-α, Mouse (P. Pastoris-expressed)

    Lane 1: 2 μg of TNF-α, Mouse (P. pastoris-expressed), reducing (R)
    > 95% as analyzed by SDS-PAGE


Background
Target Background Tumor necrosis factor alpha (TNF-α) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. Mouse TNF-α occurs as a membrane-anchored form. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and mouse TNF-α show approximately 79% homology at the amino acid level and crossreactivity between the two species.
Synonyms TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.