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Streptavidin

Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Streptavidin can bind to four moles of biotin per mole of protein,and it shows extremely high affinity, approximately 10,000-15,000 Da. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point of 6.5 to avidin's 10 far closer to that at which most useful biological interactions occur. As a result, Streptavidin frequently exhibits much lower non-specific binding than avidin does.
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Product Introduction
Purity > 95% as analyzed by SDS-PAGE
Biological Activity >15 U/mg protein, 1 U is defined as the quantity of protein that binds 1 microgramme
Expression System E. coli
Theoretical Molecular Weight 13,500 Da
Application Streptavidin can be used for the coating of solid phases (microtiter plates, beads, etc.) to immobilize or purify biotin-labeled or 2-iminobiotin-labeled molecules. Its conjugation with enzyme or fluorescein is widely used in immunological detection assays, such as the streptavidin/biotin system.
Formulation Lyophilized from a 0.22 μm filtered solution in 10 mM potassium phosphate buffer, pH 6.5-7.0.
Concentration Please refer to the COA for the specific lot.
Storage & Stability The powder may be stored at 4°C for several weeks. For long-term storage, store at -20°C.

Background
Target Background Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Streptavidin can bind to four moles of biotin per mole of protein,and it shows extremely high affinity, approximately 10,000-15,000 Da. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point of 6.5 to avidin's 10 far closer to that at which most useful biological interactions occur. As a result, Streptavidin frequently exhibits much lower non-specific binding than avidin does.
Synonyms Streptavidin

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.