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CD40L/CD154/TRAP, Human

CD40 Ligand (CD40L/CD154/TRAP) is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. It also costimulates proliferation of activated T-cell and this is accompanied by the production of IFN-γ, TNF-α, and IL2. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes.
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Product Introduction
Species Human
Protein Construction
CD40L (Met113-Leu261)
Accession # P29965
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by RP-HPLC
> 95% as analyzed by Anion-exchange FPLC
Endotoxin Level < 1 EU/μg of protein by LAL method
Biological Activity Recombinant human CD40 Ligand (CD40L/CD154/TRAP) is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5.0-10.0 ng/ml, corresponding to a Specific Activity of 1.0 x 105 IU/mg.
Expression System E. coli
Theoretical Molecular Weight 16.3 kDa
N-terminal Sequence Analysis The sequence of the first five N-terminal amino acids was determined and found to be Met-Gln-Lys-Gly-Asp.
Formulation Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0, 250 mM NaCl buffer.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile 18 MΩ-cm H₂O up to 100 µg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles.

Background
Target Background CD40 Ligand (CD40L/CD154/TRAP) is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. It also costimulates proliferation of activated T-cell and this is accompanied by the production of IFN-γ, TNF-α, and IL2. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes.
Synonyms CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154; CD40LG; TNFSF5

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.