iFluor488-Protein L
IFluor488-Protein L

VHH expression positive cell line was stained with negative control (black curve) or Protein L labeled with iFluor488 at 5 μg/mL (red curve).

IFluor488-Protein L

Lane 1: 1 μg of Protein L, reducing(R)
> 90% as analyzed by SDS-PAGE

iFluor488-Protein L

Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, which bind to the Fc region of immunoglobulins, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L.
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Overview
Species Peptostreptococcus magnus
Source E. coli
Tag His Tag
Conjugate iFluor488

Properties
Purity > 90% as analyzed by SDS-PAGE
Molecular Weight ~45 kDa, on SDS-PAGE under reducing conditions
Formulation Lyophilized from a solution in PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder in deionized water up to 0.5 mg/ml.
Storage Upon receiving, this product remains stable up to 12 months at -20°C or below. Upon reconstitution, the product can be stored for 2-3 weeks at 2-8°C or 3 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications
The optimal dilution ratio should be determined by the end user for specific applications. Flow cytometry analysis: 1:100-1:200

Examples
  • IFluor488-Protein L
    • IFluor488-Protein L

    VHH expression positive cell line was stained with negative control (black curve) or Protein L labeled with iFluor488 at 5 μg/mL (red curve).

  • IFluor488-Protein L
    • IFluor488-Protein L

    Lane 1: 1 μg of Protein L, reducing(R)
    > 90% as analyzed by SDS-PAGE


Background
Synonyms RPL; Protein L
Target Background Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, which bind to the Fc region of immunoglobulins, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L.