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PEmax MRNA V2 (Cap1, M1Ψ)

PEmax MRNA V2 (Cap1, M1Ψ)

T to A editing using PEmax system in HEK293T cells and primary T cells. X5 represents GenScript codon optimization algorithm.

PEmax mRNA V2 (Cap1, m1Ψ)

The PEmax mRNA expresses an optimized prime editor enzymatic protein, which further increased editing efficiency in synergy with PE4, PE5 and engineered pegRNAs (Chen P, et al. 2021, Cell 184, 5635-5652). After efficient delivery into cells or animals, PEmax mRNA can translate into protein including a nickase and reverse transcriptase function for gene editing in cells.
RP-A00063
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Description

The PEmax mRNA expresses an optimized prime editor enzymatic protein, which further increased editing efficiency in synergy with PE4, PE5 and engineered pegRNAs (Chen P, et al. 2021, Cell 184, 5635-5652). After efficient delivery into cells or animals, PEmax mRNA can translate into protein including a nickase and reverse transcriptase function for gene editing in cells. 
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- Codon optimized using GenScript X5 algorithm to enhance expression efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 6661 nt
Full mRNA Molecular Weight 2.16×10^6 Da
Storage buffer 1mM Sodium citrate, pH 6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect PEmax mRNA along with pegRNA and nicking sgRNA (optional) into cells through electroporation, LNP encapsulation or transfection reagent. After 3 days of incubation, extract the genomic DNA and perform PCR. Analyze the resulting PCR products by Sanger sequencing.

Examples
  • PEmax MRNA V2 (Cap1, M1Ψ)
    • PEmax MRNA V2 (Cap1, M1Ψ)

  • PEmax MRNA V2 (Cap1, M1Ψ)
    • PEmax MRNA V2 (Cap1, M1Ψ)

    T to A editing using PEmax system in HEK293T cells and primary T cells. X5 represents GenScript codon optimization algorithm.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.