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LbCas12a MRNA V2 (Cap1, M1Ψ)

LbCas12a MRNA V2 (Cap1, M1Ψ)

Editing efficiency of Cas12a on TRAC target in HEK293T cells. WT represents wildtype; X5 represents GenScript codon optimization algorithm.

LbCas12a mRNA V2 (Cap1, m1Ψ)

Cpf1 is a novel class of CRISPR-Cas DNA endonuclease. The LbCpf1/Cas12a allow efficient mutagenesis in zebrafish and Xenopus (Moreno-Mateos, et al. Nat Communications, 2017; 8: 2024). LbCpf1 increases homology-directed genome editing.
RP-A00060
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Description

Cpf1 is a novel class of CRISPR-Cas DNA endonuclease. The LbCpf1/Cas12a allow efficient mutagenesis in zebrafish and Xenopus (Moreno-Mateos, et al. Nat Communications, 2017; 8: 2024). LbCpf1 increases homology-directed genome editing.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- Codon optimized using GenScript X5 algorithm to enhance expression efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 4063 nt
Full mRNA Molecular Weight 1.32×10^6 Da
Storage buffer 1mM Sodium citrate, pH 6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 2 μg of LbCas12a mRNA and 0.4 μg of TRAC crRNA into 5×104 HEK293T cells using Lipofectamine™ MessengerMAX™ (or equivalent), on day 3, extract the genomic DNA and analyze data by NGS sequencing.

Examples
  • LbCas12a MRNA V2 (Cap1, M1Ψ)
    • LbCas12a MRNA V2 (Cap1, M1Ψ)

  • LbCas12a MRNA V2 (Cap1, M1Ψ)
    • LbCas12a MRNA V2 (Cap1, M1Ψ)

    Editing efficiency of Cas12a on TRAC target in HEK293T cells. WT represents wildtype; X5 represents GenScript codon optimization algorithm.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.