ホーム » カタログRNA＆LNP製品 » Cre mRNA (Cap1, m1Ψ)
Cre MRNA (Cap1, M1Ψ)

Cre mRNA (Cap1, m1Ψ)

*This product is under development*
Cre is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The cre-loxP-mediated recombination system is an integral experimental tool for mammalian genetic and cell biology. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudoUridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.
RP-A00005
Ask us a question
Description

Cre is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The cre-loxP-mediated recombination system is an integral experimental tool for mammalian genetic and cell biology.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 1407nt
Full mRNA Molecular Weight 459549
Storage buffer 1mM Sodium citrate, pH6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.1 ug of mRNA using 0.5 uL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of HEK293-LoxP-GFP-RFP(Bsd) cells in a 96 well plate following manufacture’s instruction. Detect the RFP expression on cells using plate reader at the excitation of 560nm, and emission at 620nm.

Examples
  • Cre MRNA (Cap1, M1Ψ)
    • Cre MRNA (Cap1, M1Ψ)


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.