HEK293/SBE-Luc
HEK293/SBE-Luc

TGF-β Responsive SBE reporter cells were plated at 37°C, 5% CO2 for 16-20 hours. The next day, TGF-β, serving as agonist, was incubated with reporter cells at 37°C, 5% CO2 for 24 hours. Luciferase substrate reagent was added and luminescence was measured by BioTek plate reader. Data were analyzed by GraphPad Prism® software.

HEK293/SBE-Luc

TGF-β Responsive SBE Reporter cell line expressing luciferase under the control of SMAD binding elements (SBE) function as the effector cells to screen the leads targeting TGF-β.
RD01131
¥2,931,390.00

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Product Introduction
Product Description TGF-β Responsive SBE Reporter cell line expressing luciferase under the control of SMAD binding elements (SBE) function as the effector cells to screen the leads targeting TGF-β.
Culture Properties Adherent
Product Type Cell line
Stability Stable through more than 30 passages with no significant changes in assay performance or expression profile.
Size 2 vials of frozen cells (>3X106 per vial in 1 ml)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium DMEM, 10% FBS, 200 μg/mL HygromycinB
Complete Growth Medium DMEM with 10% FBS
Freeze Medium-DATA 90% complete growth medium, 10% (V/V) DMSO Or OptiVitro® Cell Cryopreservation Medium

Examples
  • HEK293/SBE-Luc
    • HEK293/SBE-Luc

    TGF-β Responsive SBE reporter cells were plated at 37°C, 5% CO2 for 16-20 hours. The next day, TGF-β, serving as agonist, was incubated with reporter cells at 37°C, 5% CO2 for 24 hours. Luciferase substrate reagent was added and luminescence was measured by BioTek plate reader. Data were analyzed by GraphPad Prism® software.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.