GS-H3/RANK
GS-H3/RANK

GS-H3/RANK cells were collected using Accutase Cell Dissociation Reagent, plated and incubated at 37°C, 5% CO2 for 16-20 hours prior to addition of increasing concentration of RANKL protein. After 6 hours of incubation, luciferase substrate reagent was added and luminescence measured by PHERAStar FSX. Data were analyzed by GraphPad Prism® software.

GS-H3/RANK

GS-H3/RANK cells were collected using Accutase Cell Dissociation Reagent, plated and incubated at 37°C, 5% CO2 for 16-20 hours prior to addition of RANKL protein and increasing concentration of anti-RANK antibody. After 6 hours of incubation, luciferase substrate reagent was added and luminescence measured by PHERAStar FSX. Data were analyzed by GraphPad Prism® software.

GS-H3/RANK

The GS-H3/RANK cell line overexpresses TNF receptor superfamily member 11a (RANK) and luciferase gene driven by NF-ĸB RE. Binding of RANK ligand (RANKL) to the receptor leads to the activation of an intra- and intercellular signal transduction pathway.
RD00956
¥2,931,390.00

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Product Introduction
Product Description The GS-H3/RANK cell line overexpresses TNF receptor superfamily member 11a (RANK) and luciferase gene driven by NF-ĸB RE. Binding of RANK ligand (RANKL) to the receptor leads to the activation of an intra- and intercellular signal transduction pathway.
Culture Properties Adherent
Product Type Cell line
Size 2 vials of frozen cells (>1X106 per vial in 1 ml)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium EMEM, 10% FBS, 1 μg/mL Puromycin, 200 μg/mL Hygromycin B
Complete Growth Medium EMEM supplemented with 10% FBS
Freeze Medium-DATA 90% Complete Growth Medium (EMEM, 10% FBS), 10% DMSO

Examples
  • GS-H3/RANK
    • GS-H3/RANK

    GS-H3/RANK cells were collected using Accutase Cell Dissociation Reagent, plated and incubated at 37°C, 5% CO2 for 16-20 hours prior to addition of increasing concentration of RANKL protein. After 6 hours of incubation, luciferase substrate reagent was added and luminescence measured by PHERAStar FSX. Data were analyzed by GraphPad Prism® software.

  • GS-H3/RANK
    • GS-H3/RANK

    GS-H3/RANK cells were collected using Accutase Cell Dissociation Reagent, plated and incubated at 37°C, 5% CO2 for 16-20 hours prior to addition of RANKL protein and increasing concentration of anti-RANK antibody. After 6 hours of incubation, luciferase substrate reagent was added and luminescence measured by PHERAStar FSX. Data were analyzed by GraphPad Prism® software.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.