StrepCaptureXP™ Resin Lite

Product description

The Strep-tag® II (WSHPQFEK) and Twin-Strep-tag® (WSHPQFEKGGGSGGGSGGSAWSHPQFEK) are widely adopted affinity tags for the purification of recombinant proteins in basic research, diagnostics, and biomanufacturing.
GenScript’s StrepCaptureXP™ Resin Lite (Cat. No. L01114) is a next-generation affinity chromatography medium engineered for the efficient purification of Strep-tag® II and Twin-Strep-tag® fusion proteins. Functionalized with an innovatively engineered StrepCaptureXP ligand (a genetically optimized streptavidin variant), this resin enables high-affinity, high-specificity capture proteins from complex biological lysates, including those derived from mammalian, bacterial, and insect expression systems.
The resin enables gentle and reversible elution, helping to maximize the recovery of biologically active proteins. It is particularly suitable for applications requiring mild purification conditions, such as the purification of membrane proteins and transmembrane proteins.

Key Features

● High specificity and low background binding.
● Compatible with neutral pH buffers and gentle elution conditions.
● Excellent detergent compatibility for membrane protein purification.
● Suitable for gravity-flow chromatography, FPLC, and automated purification systems.

Selection Guide

	StrepCaptureXP Magnetic Beads (Cat. No.: L01075)	StrepCaptureXP Resin FF (Cat. No.: L01091)	StrepCaptureXP Resin Lite (Cat. No.: L01114)
Recommended Applications	High-throughput screening & small-scale purification	Scalable purification & process development	Routine research purification & small-scale protein preparation
If You Need	✓ High-throughput screening ✓ Automated parallel purification ✓ AI-driven protein screening and parallel evaluation of multiple protein candidates ✓ Fast turnaround and high first-pass success rate	✓ Scale-up purification ✓ Compatible with ÄKTA™ systems ✓ High stability for repeated purification cycles ✓ Cost-effective for long-term manufacturing and process development	✓ Flexible for small-scale protein preparation ✓ Easy-to-use format for manual purification ✓ Lower entry cost for routine research applications ✓ Challenging proteins including membrane proteins
Recommended Workflow	Automated magnetic purification platforms and parallel purification workflows	ÄKTA™ chromatography systems and scalable purification processes	Manual purification or small-scale chromatography workflows
Typical Scale	Small volume purification (Recommended: <1 mL beads equivalent)	Medium- to large-scale purification	Small-scale laboratory purification
Automation Compatibility	Compatible with automated magnetic purification platforms (e.g., AmMag™ Quatro 1300 )	Compatible with ÄKTA™ chromatography systems	Compatible with commonly used chromatography systems, including ÄKTA™ systems under recommended operating conditions (refer to Product Manual)
Binding Capacity	20–40 mg/mL beads	10–30 mg/mL resin	8–15 mg/mL resin
Reusability	≥30 purification cycles	≥100 purification cycles	≥35 purification cycles
Purity of Eluted Protein	≥90% (SDS-PAGE)	≥90% (SDS-PAGE)	≥90% (SDS-PAGE)

Properties

Formulation	50% settled resin suspended in PBS (pH 7.4) with 0.1% ProClin300
Matrix	4% highly cross-linked agarose
Average Particle Size	90 μm (45-165 μm)
Pressure	0.1 Mpa
Ligand	StrepCaptureXP
Binding Capacity	8-15 mg protein/mL settled resin. (Please note that the binding capacity is protein dependent)
Elution Method	Competitive elution with 50 mM Biotin
Reuse Capability	≥35 purification cycles when regenerated and stored as recommended.
Reagents Compatibility	Compatible with commonly used lysis reagents.
Application	purification of Strep-tag® II and Twin-Strep-tag® fusion proteins
Storage	Stored at 2°C~8°C. Do not store at 0°C or below 0°C to avoid freezing the resin.

Examples

Four Strep-tagged proteins were purified using StrepCaptureXP™ Resin Lite (GenScript, L01114) and a commercial affinity resin from Competitor-ST under identical purification conditions. Elution fractions were analyzed by SDS-PAGE.

M: Protein marker

Protein 1: AtmGFP-Strep II-Flag
Lane 1: GenScript StrepCaptureXP™ Resin Lite eluate (99.19% purity)
Lane 2: Competitor-ST eluate (79.03% purity)

Protein 2: AtmGFP-Strep II
Lane 3: GenScript StrepCaptureXP™ Resin Lite eluate (99.99% purity)
Lane 4: Competitor-ST eluate (99.99% purity)

Protein 3: Twin Strep-Brolucizumab
Lane 5: GenScript StrepCaptureXP™ Resin Lite eluate (97.13% purity)
Lane 6: Competitor-ST eluate (95.55% purity)

Protein 4: Strep-BAP-Flag
Lane 7: GenScript StrepCaptureXP™ Resin Lite eluate (98.90% purity)
Lane 8: Competitor-ST eluate (97.79% purity)

Binding capacity was compared between StrepCaptureXP™ Resin Lite (GenScript, L01114) and a commercial affinity resin from Competitor-ST using six Strep-tagged proteins expressed in E. coli and CHO cells under identical purification conditions.

SDS-PAGE analysis of HEK293-expressed ASIC2a membrane protein purification using StrepCaptureXP™ Resin Lite and a commercial affinity resin from Competitor-ST.

M: Protein marker
Lane 1: HEK293 cell lysate supernatant
Lanes 2–4: StrepCaptureXP™ Resin Lite purification (2: Flow Through; 3: Elution, 2 CV; 4: Elution, additional 3 CV)
Lanes 5–7: Competitor resin purification (5: Flow Through; 6: Elution, 2 CV; 7: Elution, additional 3 CV)
The target membrane protein (ASIC2a) has a theoretical molecular weight of 60.1 kDa.

Sample Preparation:
1. Cells were lysed in 20 mM HEPES, 150 mM NaCl (pH 7.4) containing 1–1.5% (w/v) LMNG for 2 h at 2–8°C.
2. The lysate was clarified by ultracentrifugation at 45,000 rpm for 45 min at 4°C.
3. 100 μL of resin was incubated with 12 mL of clarified membrane protein lysate for 2 h at 2–8°C.
4. After binding, washing and elution were performed according to the recommended purification protocol. Elution fractions were analyzed by SDS-PAGE.

Binding capacity of StrepCaptureXP™ Resin Lite (GenScript, L01114) was evaluated after repeated regeneration with 0.1 M NaOH. No significant loss in binding capacity was observed after 40 regeneration cycles.

Terms of Use

Terms of Use

Product is provided under a free, non-transferable, limited license to use the Product solely in the manner described in its accompanying Product documentation (including, without limitation, any instructions for use, user manuals, certificates of analysis (CoA), datasheets, labels, and other written or electronic materials supplied or referenced by GenScript) and solely for the stated purpose. Any other use may require a separate license from GenScript. This limited, non-transferable license is granted upon the purchase of the Product from GenScript or its authorized distributors.
This provision shall not be construed as a warranty of merchantability or fitness for a particular purpose or commercial use.
Any reproduction, distribution, modification, reverse engineering, cloning, reverse cloning, sequence determination, sublicensing, or use of the Product to develop competing products or services is prohibited without GenScript’s prior written consent.

Third-Party Trademark Acknowledgement
All product and company names mentioned in this manual are trademarks or registered trademarks of their respective owners, including but not limited to: Strep-tag and Twin-Strep-tag, trademarks of IBA GmbH; ÄKTA, a trademark of Global Life Sciences Solutions USA LLC and its affiliated companies doing business as Cytiva; Triton, a trademark of The Dow Chemical Company; and Tween, a trademark of Croda International Plc. These trademarks are used solely for the purpose of identifying compatible reagents, products, or technologies. GenScript is not sponsored by, affiliated with, or endorsed by the owners of these trademarks.

For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.

Cat. No.	L01114
Size	1 ml Resin (Total 2 ml) : Quote 5 ml Resin (Total 10 ml) : Quote 10 ml Resin (Total 20 ml) : Quote 25 ml Resin (Total 50 ml) : Quote Buy in bulk
Quantity
TAT
Price	¥9,165.00


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StrepCaptureXP™ Resin Lite

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