Protein Isolation and Analysis » cGMP-HRP
CGMP-HRP

cGMP Competitive ELISA
1.ELISA plate was coated with goat anti mouse IgG antibody
2.Anti-cGMP monoclonal antibody (Cat. No. A01508) at appropriate dilution and cGMP standards or testing compounds were added into appropriate reaction wells.
3.After a period of incubation, HRP-cGMP conjugate (Cat.No.M01058) was added followed by proper period of incubation.
4.ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature.
5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.

cGMP-HRP

cGMP-peroxidase conjugate is a product most suitable for GenScript's THETM Anti cGMP monoclonal antibody (Cat. No. A01508) and Rabbit Anti-cGMP polyclonal antibody (Cat. No. A00615) in immunoassay development for cGMP detection. HRP is conjugated to the C-8 position of cGMP.
M01058
¥20,300.00

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Properties
Concentration Phosphate buffered saline, pH 7.4, containing 1% BSA and 0.01% thimerosal
Storage The conjugate can be stored for up to two years at -20°C or below.

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by the antibody affinity, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this product for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000 - 1:20,000
Other: user-optimized

Examples
  • CGMP-HRP
    • CGMP-HRP

    cGMP Competitive ELISA
    1.ELISA plate was coated with goat anti mouse IgG antibody
    2.Anti-cGMP monoclonal antibody (Cat. No. A01508) at appropriate dilution and cGMP standards or testing compounds were added into appropriate reaction wells.
    3.After a period of incubation, HRP-cGMP conjugate (Cat.No.M01058) was added followed by proper period of incubation.
    4.ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature.
    5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.


Background
Target Background cGMP-peroxidase conjugate is a product most suitable for GenScript's THETM Anti cGMP monoclonal antibody (Cat. No. A01508) and Rabbit Anti-cGMP polyclonal antibody (Cat. No. A00615) in immunoassay development for cGMP detection. HRP is conjugated to the C-8 position of cGMP.