安定細胞株製品 » Human Recombinant CHO-K1/ACE2 Stable Cell Line
CHO-K1/ACE2 Stable Cell Line

Figure 1: FACS analysis of cell surface expression of ACE2 on CHO-K1 cells.
CHO-K1/ACE2 cells (green) and negative control CHO-K1 cells (blue) were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

CHO-K1/ACE2 Stable Cell Line

Figure 2: FACS analysis of cell surface expression of ACE2 on CHO-K1 cells from different passages.
The CHO-K1/ACE2 cells and the negative control CHO-K1 cells were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P6, P11, P16 and P20 represent cell passage numbers.

CHO-K1/ACE2 Stable Cell Line

Recombinant CHO-K1 cells stably express Angiotensin-converting enzyme 2 (ACE2) on the cell surface. The surface expression of ACE2 is validated by FACS analysis. This stable cell line product is designed for cell-based binding assays that measure binding affinity and stability of antibody based biologics binding with ACE2.
M00771
¥1,424,115.00

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Overview
Product Description Recombinant CHO-K1 cells stably express Angiotensin-converting enzyme 2 (ACE2) on the cell surface. The surface expression of ACE2 is validated by FACS analysis. This stable cell line product is designed for cell-based binding assays that measure binding affinity and stability of antibody based biologics binding with ACE2. 
Applications Cell-based binding assay; use as immunogen for antibody development. 

Product Introduction
Expressed Gene Codon Optimized from NM_021804.2; no expressed tags
Target Protein NP_068576.1
Host Cell CHO-K1
Quantity 2 vials of frozen cells (>1X106 per vial in 1 ml)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

Culture Conditions
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium F-12K (Cat. # 21127-022, Gibco), 10% FBS (Cat. #10099-141, Gibco)
Culture Medium F-12K, 10% FBS, 250 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)

Background
Gene Synonyms Angiotensin-converting enzyme 2; ACEH
Background Angiotensin-converting enzyme 2 (ACE2), discovered as a homologue of ACE, acts as its physiological counterbalance providing homeostatic regulation of circulating angiotensin II (Ang II) levels. ACE2 is a zinc metalloenzyme and carboxypeptidase located as an ectoenzyme on the surface of endothelial and other cells. While its primary substrate appears to be Ang II, it can hydrolyze a number of other physiological substrates. Additionally, ACE2 functions in other noncatalytic cellular roles including the regulation of intestinal neutral amino acid transport. It also serendipitously acts as the receptor for the severe acute respiratory syndrome virus. Upregulation of ACE2 expression and function is increasingly recognized as a potential therapeutic strategy in hypertension and cardiovascular disease, diabetes, lung injury, and fibrotic disorders. ACE2 is regulated at multiple levels including transcriptional, posttranscriptional (miRNA and epigenetic), and posttranslational through its shedding from the cell surface.

Examples
  • CHO-K1/ACE2 Stable Cell Line
    • CHO-K1/ACE2 Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of ACE2 on CHO-K1 cells.
    CHO-K1/ACE2 cells (green) and negative control CHO-K1 cells (blue) were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

  • CHO-K1/ACE2 Stable Cell Line
    • CHO-K1/ACE2 Stable Cell Line

    Figure 2: FACS analysis of cell surface expression of ACE2 on CHO-K1 cells from different passages.
    The CHO-K1/ACE2 cells and the negative control CHO-K1 cells were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P6, P11, P16 and P20 represent cell passage numbers.