Figure 1. FACS analysis of cell surface expression of ACE2 on CHO-K1 cells. CHO-K1/ACE2 cells (green) and negative control CHO-K1 cells (blue) were stained with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

Figure 2. FACS analysis of cell surface expression of ACE2 on CHO-K1 cells of different passages. The CHO-K1/ACE2 cells and the negative control CHO-K1 cells were stained with Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P6, P11, P16 and P20 are cell passage numbers.

Figure 1. FACS analysis of cell surface expression of ACE2 on CHO-K1 cells. CHO-K1/ACE2 cells (green) and negative control CHO-K1 cells (blue) were stained with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

Figure 2. FACS analysis of cell surface expression of ACE2 on CHO-K1 cells of different passages. The CHO-K1/ACE2 cells and the negative control CHO-K1 cells were stained with Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P6, P11, P16 and P20 are cell passage numbers.

CHO-K1/ACE2 Stable Cell Line

Recombinant CHO-K1 cells stably express Angiotensin-converting enzyme 2 (ACE2) on the cell surface. The surface expression of ACE2 is validated by FACS analysis. This stable cell line product is designed for cell-based binding assays that measure binding affinity and stability of antibody based biologics binding with ACE2.

Cat. No.	M00771
Size	2 vials : Quote Buy in bulk
Quantity
Price	¥1,465,695.00


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Product Introduction

Product Description	Recombinant CHO-K1 cells stably express Angiotensin-converting enzyme 2 (ACE2) on the cell surface. The surface expression of ACE2 is validated by FACS analysis. This stable cell line product is designed for cell-based binding assays that measure the binding affinity and stability of antibody based biologics binding with ACE2.
Culture Properties	Adherent
Stability	Stability: Stable through more than 20 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions

Culture Medium	F-12K (Kaighn’s), 10% FBS, 250 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium	F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)

Examples

Figure 1. FACS analysis of cell surface expression of ACE2 on CHO-K1 cells. CHO-K1/ACE2 cells (green) and negative control CHO-K1 cells (blue) were stained with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

Figure 2. FACS analysis of cell surface expression of ACE2 on CHO-K1 cells of different passages. The CHO-K1/ACE2 cells and the negative control CHO-K1 cells were stained with Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P6, P11, P16 and P20 are cell passage numbers.

Figure 1. FACS analysis of cell surface expression of ACE2 on CHO-K1 cells. CHO-K1/ACE2 cells (green) and negative control CHO-K1 cells (blue) were stained with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

Figure 2. FACS analysis of cell surface expression of ACE2 on CHO-K1 cells of different passages. The CHO-K1/ACE2 cells and the negative control CHO-K1 cells were stained with Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P6, P11, P16 and P20 are cell passage numbers.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

CHO-K1/ACE2 Stable Cell Line

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