![HEK293/ACE2 Stable Cell Line HEK293/ACE2 Stable Cell Line](/product/documents/down?doc_name=M00770_FACS Analysis.png&file=scm_files/productFile_notes/2022/04/08/63343422-7458-40f1-82f2-153aef99f226.png)
Figure 1: FACS analysis of cell surface expression of ACE2 on HEK293 cells.
HEK293/ACE2 cells (red) and negative control HEK293 cells (green) were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).
![HEK293/ACE2 Stable Cell Line HEK293/ACE2 Stable Cell Line](/product/documents/down?doc_name=M00770_Binding Assay.png&file=scm_files/productFile_notes/2022/04/08/7aaedc61-8848-48fb-8e27-05292bb97e86.png)
Figure 2: Cell-based binding assay of SARS-CoV-2 spike protein RBD with HEK293/ACE2 cells.
HEK293/ACE2 cells were incubated with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript) in serial dilution. Then the cells were stained with THE™ His Tag Antibody [iFluor 488], mAb, Mouse (Cat. No. A01800, GenScript) and analyzed with flow cytometer. FACS analysis shows the EC50 of spike protein RBD binding with ACE2 on HEK293 cells is 1.071 ug/ml.
![HEK293/ACE2 Stable Cell Line HEK293/ACE2 Stable Cell Line](/product/documents/down?doc_name=M00770_Neutralization Assay.png&file=scm_files/productFile_notes/2022/04/08/30a8c14d-c21d-4df5-8f33-aca6ec3df2b9.png)
FACS analysis histogram shows ACE2 expression in HEK293/ACE2 stable cell line (Cat. No. M00770) compared with negative control. HEK293/ACE2 cells are plated at 3E5+ cells /well in 100ul PBS in 96-well plate, incubated with 10 µg/ml S-RBD-His (GenScript, Cat. No. Z03479) on ice for 1 hour, or left untreated. Both groups are then washed with PBS, incubated with Anti-His 2nd antibody (GenScript, Cat. No. A01800) at 1:200 on ice for 1 hour, washed with PBS, and then analyzed on flow cytometer.
![HEK293/ACE2 Stable Cell Line HEK293/ACE2 Stable Cell Line](/product/documents/down?doc_name=M00770_Stability Validation.png&file=scm_files/productFile_notes/2022/04/08/15b5ed0b-d988-4c42-b507-01c5ba1ad380.png)
Figure 5: FACS analysis of cell surface expression of ACE2 on HEK293 cells from different passages.
The HEK293/ACE2 cells and the negative control HEK293 cells were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P8, P13, P17 and P21 represent cell passage numbers.
![HEK293/ACE2 Stable Cell Line HEK293/ACE2 Stable Cell Line](/product/documents/down?doc_name=M00770_Pseudovirus Transfection Assay.png&file=scm_files/productFile_notes/2022/04/14/acb4e282-3084-4b89-bbe4-fbb48a83fca3.png)
Figure 4: SARS-CoV-2 (B.1.1.529, Omicron) pseudovirus transfection on HEK293/ACE2 cells.
SARS-CoV-2 pseudovirus with S glycoprotein as the envelope protein. The pseudovirus entry was initiated by recognition and binding between RBD and ACE2 on HEK293 cells
HEK293/ACE2 Stable Cell Line
M00770 | |
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