安定細胞株製品 » Human Recombinant ACE2 Stable Cell Line
HEK293/ACE2 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of ACE2 on HEK293 cells. HEK293/ACE2 cells (red) and negative control HEK293 cells (green) were stained with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

HEK293/ACE2 Stable Cell Line

Figure 2. Cell-based binding assay of SARS-CoV-2 spike protein RBD with HEK293/ACE2 cells. HEK293/ACE2 cells were incubated with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript) in serial dilution. Then the cells were stained with THE™ His Tag Antibody [iFluor 488] Mouse (Cat. No. A01800, GenScript) and analyzed with flow cytometer. FACS analysis shows the EC50 of spike protein RBD binding with ACE2 on HEK293 cells is 1.07 ug/ml.

HEK293/ACE2 Stable Cell Line

Figure 3. Neutralization assay of SARS-CoV-2 spike protein RBD by ACE2-Fc fusion proteins on HEK293/ACE2 cells. HEK293/ACE2 cells were incubated with 1 µg/ml SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript) and ACE2-Fc fusion protein (Cat. No. Z03484, GenScript) in serial dilution. Then the cells were stained with THE™ His Tag Antibody [iFluor 488], (Cat. No. A01800, GenScript) and analyzed with flow cytometer.

HEK293/ACE2 Stable Cell Line

Figure 4. SARS-CoV-2 (B.1.1.529) pseudovirus infection on HEK293/ACE2 cells. SARS-CoV-2 pseudovirus is prepared with S glycoprotein as the envelope protein. The pseudovirus entry was initiated by recognition and binding between S glycoprotein and ACE2 on HEK293 cells.

HEK293/ACE2 Stable Cell Line

Figure 5. FACS analysis of cell surface expression of ACE2 on HEK293 cells of different passages. The HEK293/ACE2 cells and the negative control HEK293 cells were stained with Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P8, P13, P17 and P21 are cell passage numbers.

HEK293/ACE2 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of ACE2 on HEK293 cells. HEK293/ACE2 cells (red) and negative control HEK293 cells (green) were stained with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

HEK293/ACE2 Stable Cell Line

Figure 2. Cell-based binding assay of SARS-CoV-2 spike protein RBD with HEK293/ACE2 cells. HEK293/ACE2 cells were incubated with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript) in serial dilution. Then the cells were stained with THE™ His Tag Antibody [iFluor 488] Mouse (Cat. No. A01800, GenScript) and analyzed with flow cytometer. FACS analysis shows the EC50 of spike protein RBD binding with ACE2 on HEK293 cells is 1.07 ug/ml.

HEK293/ACE2 Stable Cell Line

Figure 3. Neutralization assay of SARS-CoV-2 spike protein RBD by ACE2-Fc fusion proteins on HEK293/ACE2 cells. HEK293/ACE2 cells were incubated with 1 µg/ml SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript) and ACE2-Fc fusion protein (Cat. No. Z03484, GenScript) in serial dilution. Then the cells were stained with THE™ His Tag Antibody [iFluor 488], (Cat. No. A01800, GenScript) and analyzed with flow cytometer.

HEK293/ACE2 Stable Cell Line

Figure 4. SARS-CoV-2 (B.1.1.529) pseudovirus infection on HEK293/ACE2 cells. SARS-CoV-2 pseudovirus is prepared with S glycoprotein as the envelope protein. The pseudovirus entry was initiated by recognition and binding between S glycoprotein and ACE2 on HEK293 cells.

HEK293/ACE2 Stable Cell Line

Figure 5. FACS analysis of cell surface expression of ACE2 on HEK293 cells of different passages. The HEK293/ACE2 cells and the negative control HEK293 cells were stained with Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P8, P13, P17 and P21 are cell passage numbers.

HEK293/ACE2 Stable Cell Line

Recombinant HEK293 cells stably express Angiotensin-converting enzyme 2 (ACE2) on the cell surface. The surface expression of ACE2 is validated by FACS analysis. This stable cell line product is designed for cell-based binding assays that measure the binding affinity and stability of antibody based biologics binding with ACE2, and can be utilized for SARS-Cov-2 pseudovirus neutralization assay for screening neutralizing antibodies.
M00770
¥1,465,695.00

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Product Introduction
Product Description Recombinant HEK293 cells stably express Angiotensin-converting enzyme 2 (ACE2) on the cell surface. The surface expression of ACE2 is validated by FACS analysis. This stable cell line product is designed for cell-based binding assays that measure the binding affinity and stability of antibody based biologics binding with ACE2, and can be utilized for SARS-Cov-2 pseudovirus neutralization assay for screening neutralizing antibodies.
Culture Properties Adherent.
Stability Stability: Stable through more than 20 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Culture Medium: DMEM, 10% FBS, 250 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies).
Complete Growth Medium DMEM (Cat. No. 10569, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma).

Examples
  • HEK293/ACE2 Stable Cell Line
    • HEK293/ACE2 Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of ACE2 on HEK293 cells. HEK293/ACE2 cells (red) and negative control HEK293 cells (green) were stained with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

  • HEK293/ACE2 Stable Cell Line
    • HEK293/ACE2 Stable Cell Line

    Figure 2. Cell-based binding assay of SARS-CoV-2 spike protein RBD with HEK293/ACE2 cells. HEK293/ACE2 cells were incubated with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript) in serial dilution. Then the cells were stained with THE™ His Tag Antibody [iFluor 488] Mouse (Cat. No. A01800, GenScript) and analyzed with flow cytometer. FACS analysis shows the EC50 of spike protein RBD binding with ACE2 on HEK293 cells is 1.07 ug/ml.

  • HEK293/ACE2 Stable Cell Line
    • HEK293/ACE2 Stable Cell Line

    Figure 3. Neutralization assay of SARS-CoV-2 spike protein RBD by ACE2-Fc fusion proteins on HEK293/ACE2 cells. HEK293/ACE2 cells were incubated with 1 µg/ml SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript) and ACE2-Fc fusion protein (Cat. No. Z03484, GenScript) in serial dilution. Then the cells were stained with THE™ His Tag Antibody [iFluor 488], (Cat. No. A01800, GenScript) and analyzed with flow cytometer.

  • HEK293/ACE2 Stable Cell Line
    • HEK293/ACE2 Stable Cell Line

    Figure 4. SARS-CoV-2 (B.1.1.529) pseudovirus infection on HEK293/ACE2 cells. SARS-CoV-2 pseudovirus is prepared with S glycoprotein as the envelope protein. The pseudovirus entry was initiated by recognition and binding between S glycoprotein and ACE2 on HEK293 cells.

  • HEK293/ACE2 Stable Cell Line
    • HEK293/ACE2 Stable Cell Line

    Figure 5. FACS analysis of cell surface expression of ACE2 on HEK293 cells of different passages. The HEK293/ACE2 cells and the negative control HEK293 cells were stained with Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P8, P13, P17 and P21 are cell passage numbers.

  • HEK293/ACE2 Stable Cell Line
    • HEK293/ACE2 Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of ACE2 on HEK293 cells. HEK293/ACE2 cells (red) and negative control HEK293 cells (green) were stained with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

  • HEK293/ACE2 Stable Cell Line
    • HEK293/ACE2 Stable Cell Line

    Figure 2. Cell-based binding assay of SARS-CoV-2 spike protein RBD with HEK293/ACE2 cells. HEK293/ACE2 cells were incubated with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript) in serial dilution. Then the cells were stained with THE™ His Tag Antibody [iFluor 488] Mouse (Cat. No. A01800, GenScript) and analyzed with flow cytometer. FACS analysis shows the EC50 of spike protein RBD binding with ACE2 on HEK293 cells is 1.07 ug/ml.

  • HEK293/ACE2 Stable Cell Line
    • HEK293/ACE2 Stable Cell Line

    Figure 3. Neutralization assay of SARS-CoV-2 spike protein RBD by ACE2-Fc fusion proteins on HEK293/ACE2 cells. HEK293/ACE2 cells were incubated with 1 µg/ml SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript) and ACE2-Fc fusion protein (Cat. No. Z03484, GenScript) in serial dilution. Then the cells were stained with THE™ His Tag Antibody [iFluor 488], (Cat. No. A01800, GenScript) and analyzed with flow cytometer.

  • HEK293/ACE2 Stable Cell Line
    • HEK293/ACE2 Stable Cell Line

    Figure 4. SARS-CoV-2 (B.1.1.529) pseudovirus infection on HEK293/ACE2 cells. SARS-CoV-2 pseudovirus is prepared with S glycoprotein as the envelope protein. The pseudovirus entry was initiated by recognition and binding between S glycoprotein and ACE2 on HEK293 cells.

  • HEK293/ACE2 Stable Cell Line
    • HEK293/ACE2 Stable Cell Line

    Figure 5. FACS analysis of cell surface expression of ACE2 on HEK293 cells of different passages. The HEK293/ACE2 cells and the negative control HEK293 cells were stained with Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P8, P13, P17 and P21 are cell passage numbers.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.