安定細胞株製品 » Cyno Recombinant CD47 Stable Cell Line
CHO-K1/Cyno CD47 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of cynomolgus CD47 on CHO-K1/cyno CD47 cells. The CHO-K1/cyno CD47 cells (blue) and the negative control CHO-K1 cells (red) were probed using human anti-CD47 antibody (Hu5F9) (Cat. No. B3048, BIOINTRON) and Alexa FluorTM 488 conjugated Alexa FluorTM 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).

CHO-K1/Cyno CD47 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of cynomolgus CD47 on CHO-K1/cyno CD47 cells. The CHO-K1/cyno CD47 cells (blue) and the negative control CHO-K1 cells (red) were probed using human anti-CD47 antibody (Hu5F9) (Cat. No. B3048, BIOINTRON) and Alexa FluorTM 488 conjugated Alexa FluorTM 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).

CHO-K1/Cyno CD47 Stable Cell Line

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue, distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.
M00632
¥1,661,121.00

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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress cynomolgus CD47 on the surface. The surface expression of CD47 on this cell is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screening antibodies against CD47 or to measure binding affinity between CD47 and anti-CD47 antibodies.
Culture Properties Adherent.
Stability Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies).
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

Examples
  • CHO-K1/Cyno CD47 Stable Cell Line
    • CHO-K1/Cyno CD47 Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of cynomolgus CD47 on CHO-K1/cyno CD47 cells. The CHO-K1/cyno CD47 cells (blue) and the negative control CHO-K1 cells (red) were probed using human anti-CD47 antibody (Hu5F9) (Cat. No. B3048, BIOINTRON) and Alexa FluorTM 488 conjugated Alexa FluorTM 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).

  • CHO-K1/Cyno CD47 Stable Cell Line
    • CHO-K1/Cyno CD47 Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of cynomolgus CD47 on CHO-K1/cyno CD47 cells. The CHO-K1/cyno CD47 cells (blue) and the negative control CHO-K1 cells (red) were probed using human anti-CD47 antibody (Hu5F9) (Cat. No. B3048, BIOINTRON) and Alexa FluorTM 488 conjugated Alexa FluorTM 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.