安定細胞株製品 » Human Recombinant FcRn Stable Cell Line
CHO-K1/FcRn Stable Cell Line

Figure 1. FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells. The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were probed using FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz Biotechnology) and PE conjugated Goat anti-mouse IgG (minimal x-reactivity) antibody (Cat. No. 405307, Biolegend).

CHO-K1/FcRn Stable Cell Line

Figure 1. FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells. The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were probed using FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz Biotechnology) and PE conjugated Goat anti-mouse IgG (minimal x-reactivity) antibody (Cat. No. 405307, Biolegend).

CHO-K1/FcRn Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human FcRn and B2M subunit on the surface. The B2M subunits enable stable expression of FcRn in cells. Association of FcRn alpha-chain with beta (2) m is also important for IgG binding. The surface expression of FcRn is validated by FACS analysis.
M00603
¥1,744,177.00

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Product Introduction
Product Description Recombinant CHO - K1 cells stably overexpress human FcRn and B2M subunit on the surface. The B2M subunits enable stable expression of FcRn in cells. Association of FcRn alpha - chain with beta (2) m is also important for IgG binding. The surface expression of FcRn is validated by FACS analysis.
Culture Properties Adherent.
Stability Stable through more than 15 passages without significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 200 μg/ml Hygromycin B (Cat. No.10687010, Life Technologies).
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. A5669701, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma).

Examples
  • CHO-K1/FcRn Stable Cell Line
    • CHO-K1/FcRn Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells. The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were probed using FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz Biotechnology) and PE conjugated Goat anti-mouse IgG (minimal x-reactivity) antibody (Cat. No. 405307, Biolegend).

  • CHO-K1/FcRn Stable Cell Line
    • CHO-K1/FcRn Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells. The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were probed using FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz Biotechnology) and PE conjugated Goat anti-mouse IgG (minimal x-reactivity) antibody (Cat. No. 405307, Biolegend).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.