安定細胞株製品 » Human Recombinant CD32B 232Thr Stable Cell Line
CHO-K1/CD32B 232Thr Stable Cell Line

Figure 1: FACS analysis of cell surface expression of CD32B 232 Thr on CHO-K1/CD32B 232 Thr cells.
The CHO-K1/CD32B 232 Thr cells (blue) and the negative control CHO-K1 cells (red) were stained with APC anti-human CD32 antibody (Cat. No. 303208, BioLegend).

CHO-K1/CD32B 232Thr Stable Cell Line

Recombinant CHO-K1 cells stably overexpress low affinity immunoglobulin gamma Fc region receptor II-b (FcγRIIb / CD32B 232Thr). The surface expression of CD32B 232Thr is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD32B 232Ile. GenScript also offers CD32B 232Ile overexpression stable cell line (Cat. No. M00587) for FcγRIIb polymorphism study.
M00600
¥1,694,697.00

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Overview
Product Description Recombinant CHO-K1 cells stably overexpress low affinity immunoglobulin gamma Fc region receptor II-b (FcγRIIb / CD32B 232Thr). The surface expression of CD32B 232Thr is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD32B 232Ile. GenScript also offers CD32B 232Ile overexpression stable cell line (Cat. No. M00587) for FcγRIIb polymorphism study.
Applications Cell-based binding assay; use as immunogen for antibody development

Product Introduction
Expressed Gene Codon optimized from KR710627.1; no expressed tags
Target Protein AKI71009.1
Host Cell CHO-K1
Quantity 2 vials of frozen cells (>1×106 per vial in 1 ml) 
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

Culture Conditions
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham's F-12K (Kaighn's) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco)
Culture Medium Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No.A11138-03, Gibco)

Background
Gene Synonyms CD32; FCG2; CD32B; FCGR2; IGFR2
Background FCGR2B (CD32B) is a low affinity receptor for Immunoglobulin G (IgG). Mutation in the gene in humans leads to a lupus phenotype. The cytoplasmic part of this receptor contains an immunoreceptor tyrosine-based inhibitory motif, in contrast to the activating isoform, FCGR2A (CD32A).

Examples
  • CHO-K1/CD32B 232Thr Stable Cell Line
    • CHO-K1/CD32B 232Thr Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of CD32B 232 Thr on CHO-K1/CD32B 232 Thr cells.
    The CHO-K1/CD32B 232 Thr cells (blue) and the negative control CHO-K1 cells (red) were stained with APC anti-human CD32 antibody (Cat. No. 303208, BioLegend).