安定細胞株製品 » Human Recombinant CD32A 131His Stable Cell Line
CHO-K1/human CD32A 131His Stable Cell Line

Figure 1: FACS analysis of cell surface expression of human CD32A 131His on CHO-K1 cells.
CHO-K1/human CD32A 131His cells (blue) and negative control CHO-K1 cells (red) were stained with APC Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Bioscience).

CHO-K1/human CD32A 131His Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human immunoglobulin gamma Fc region receptor II-a (FcγRIIa / CD32A 131His). The surface expression of CD32A 131His is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32A 131His. GenScript also offers CD32A 131Arg stable cell line (Cat. No. M00887) for FcγRIIa polymorphism study.
M00598
¥1,694,697.00

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Overview
Product Description Recombinant CHO-K1 cells stably overexpress human immunoglobulin gamma Fc region receptor II-a (FcγRIIa / CD32A 131His). The surface expression of CD32A 131His is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32A 131His. GenScript also offers CD32A 131Arg stable cell line (Cat. No. M00887) for FcγRIIa polymorphism study.
Applications Cell-based binding assay; use as immunogen for antibody development

Product Introduction
Expressed Gene Codon optimized from NM_001136219.1; no expressed tags
Target Protein NP_001129691.1
Host Cell CHO-K1
Quantity Two vials of frozen cells (>1×106 per vial in 1 ml)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

Culture Conditions
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

Background
Gene Synonyms CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
Background CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loop acts to lower the production of IgG by B cells when there is a surplus in the body. Monoclonal antibodies can distinguish between CD32A and CD32B.

Examples
  • CHO-K1/human CD32A 131His Stable Cell Line
    • CHO-K1/human CD32A 131His Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of human CD32A 131His on CHO-K1 cells.
    CHO-K1/human CD32A 131His cells (blue) and negative control CHO-K1 cells (red) were stained with APC Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Bioscience).