安定細胞株製品 » Human Recombinant CD16A 158V Stable Cell Line
CHO-K1/CD16A 158V Stable Cell Line

Figure 1. FACS analysis of cell surface expression of CD16A 158V on CHO-K1/CD16A 158V cells. The CHO-K1/CD16A 158V cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-CyTM5 mouse anti-human CD16 (Cat. No. 555408, BD Bioscience).

CHO-K1/CD16A 158V Stable Cell Line

Figure 1. FACS analysis of cell surface expression of CD16A 158V on CHO-K1/CD16A 158V cells. The CHO-K1/CD16A 158V cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-CyTM5 mouse anti-human CD16 (Cat. No. 555408, BD Bioscience).

CHO-K1/CD16A 158V Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human low affinity immunoglobulin gamma Fc region receptor III-A (FcγRIIIa/CD16A 158V) and Fc epsilon receptor Ig (FcER1G) on the surface. The surface expression of CD16A 158V is validated by FACS analysis.
M00597
¥1,744,177.00

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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress human low affinity immunoglobulin gamma Fc region receptor III-A (FcγRIIIa/CD16A 158V) and Fc epsilon receptor Ig (FcER1G) on the surface. The surface expression of CD16A 158V is validated by FACS analysis.
Culture Properties Adherent.
Stability Stable through more than 15 passages without significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No.10687010, Life Technologies).
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma).

Examples
  • CHO-K1/CD16A 158V Stable Cell Line
    • CHO-K1/CD16A 158V Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of CD16A 158V on CHO-K1/CD16A 158V cells. The CHO-K1/CD16A 158V cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-CyTM5 mouse anti-human CD16 (Cat. No. 555408, BD Bioscience).

  • CHO-K1/CD16A 158V Stable Cell Line
    • CHO-K1/CD16A 158V Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of CD16A 158V on CHO-K1/CD16A 158V cells. The CHO-K1/CD16A 158V cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-CyTM5 mouse anti-human CD16 (Cat. No. 555408, BD Bioscience).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.