安定細胞株製品 » Human Recombinant CD47 Stable Cell Line
CHO-K1/human CD47 Stable Cell Line

Figure 1: FACS analysis of cell surface expression of CD47 on CHO-K1/CD47 cells. The CHO-K1/CD47 cells (Green) and the negative control CHO-K1 cells (Red) were probed using Human anti-CD47 antibody (Hu5F9) (Cat. No. B3048, BIOINTRON) and Alexa Fluor™ 488 conjugated Alexa Fluor™ 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).

CHO-K1/human CD47 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human CD47 on the surface. The surface expression of CD47 on this cell is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screening antibodies against CD47 or to measure binding affinity between CD47 and anti-CD47 antibodies.
M00581
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Overview
Product Description Recombinant CHO-K1 cells stably overexpress human CD47 on the surface. The surface expression of CD47 on this cell is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screening antibodies against CD47 or to measure binding affinity between CD47 and anti-CD47 antibodies.
Applications Binding assay or use as immunogen

Product Introduction
Expressed Gene NCBI reference sequence NM_001777.2; no expressed tags
Target Protein NP_001768.1
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages without significant changes in assay performance or expression profile.

Culture Conditions
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)

Background
Gene Synonyms IAP, MER6, OA3
Background CD47 (Cluster of Differentiation 47), also known as integrin-associated protein (IAP), is a transmembrane protein that in humans is encoded by CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).
CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. CD47 is ubiquitously expressed in human cells and has been found to be overexpressed in many different tumor cells.

Examples
  • CHO-K1/human CD47 Stable Cell Line
    • CHO-K1/human CD47 Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of CD47 on CHO-K1/CD47 cells. The CHO-K1/CD47 cells (Green) and the negative control CHO-K1 cells (Red) were probed using Human anti-CD47 antibody (Hu5F9) (Cat. No. B3048, BIOINTRON) and Alexa Fluor™ 488 conjugated Alexa Fluor™ 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.