安定細胞株製品 » Human Recombinant CD47 Stable Cell Line
CHO-K1/human CD47 Stable Cell Line

Figure 1: FACS analysis of cell surface expression of CD47 on CHO-K1/CD47 cells. The CHO-K1/CD47 cells (Green) and the negative control CHO-K1 cells (Red) were probed using Human anti-CD47 antibody (Hu5F9) (Cat. No. B3048, BIOINTRON) and Alexa Fluor™ 488 conjugated Alexa Fluor™ 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).

CHO-K1/human CD47 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress Homo sapiens CD47 molecule (CD47) on the cell surface. The surface expression of CD47 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screening antibodies against CD47 or to measure binding affinity between CD47 and anti-CD47 antibodies.
M00581
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Overview
Product Description Recombinant CHO-K1 cells stably overexpress Homo sapiens CD47 molecule (CD47) on the cell surface. The surface expression of CD47 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screening antibodies against CD47 or to measure binding affinity between CD47 and anti-CD47 antibodies. 
Applications Cell-based binding assay or use as immunogen

Product Introduction
Expressed Gene Codon optimized from NM_001777.3; no expressed tags
Target Protein NP_001768.1
Host Cell CHO-K1
Size 2 vials of frozen cells (>1×106 per vial in 1 ml) 
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 15 passages with no significant changes in assay performance or expression profile. 

Culture Conditions
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% DMSO (Cat. No.D2650, Sigma)
Complete Growth Medium Ham's F-12K (Kaighn's) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium Ham's F-12K (Kaighn's) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 4 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

Background
Gene Synonyms IAP, MER6, OA3
Background CD47 (Cluster of Differentiation 47), also known as integrin-associated protein (IAP), is a transmembrane protein that in humans is encoded by CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).
CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. CD47 is ubiquitously expressed in human cells and has been found to be overexpressed in many different tumor cells.

Examples
  • CHO-K1/human CD47 Stable Cell Line
    • CHO-K1/human CD47 Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of CD47 on CHO-K1/CD47 cells. The CHO-K1/CD47 cells (Green) and the negative control CHO-K1 cells (Red) were probed using Human anti-CD47 antibody (Hu5F9) (Cat. No. B3048, BIOINTRON) and Alexa Fluor™ 488 conjugated Alexa Fluor™ 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).