Mouse Recombinant Adenosine A2A Receptor Stable Cell Line
CHO-K1/mouse ADORA2A Stable Cell Line

Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with NECA in CHO-K1/mouse ADORA2A cells. d2 acceptor fluorophore -labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/mouse ADORA2A cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of NECA on CHO-K1/mouse ADORA2A cells was 42.55 nM.

CHO-K1/mouse ADORA2A Stable Cell Line

Recombinant CHO-K1 cells stably overexpress Mus musculus adenosine A2A receptor (ADORA2A) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
M00575
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Product Introduction
Product Description Recombinant CHO-K1 cells stably overexpress Mus musculus adenosine A2A receptor (ADORA2A) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Conditions
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

Examples
  • CHO-K1/mouse ADORA2A Stable Cell Line
    • CHO-K1/mouse ADORA2A Stable Cell Line

    Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with NECA in CHO-K1/mouse ADORA2A cells. d2 acceptor fluorophore -labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/mouse ADORA2A cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of NECA on CHO-K1/mouse ADORA2A cells was 42.55 nM.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.