安定細胞株製品 » Cyno Recombinant PD-1 Stable Cell Line
CHO-K1/cyno PD-1 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of cynomolgus PD-1 on CHO-K1/cyno PD-1 cells. The CHO-K1/cyno PD-1 cells (red) and the negative control CHO-K1 cells (blue) were probed using APC-conjugated anti-human CD279 antibody (Cat. No. 329908, Biolegend).

CHO-K1/cyno PD-1 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress cynomolgus programmed cell death protein 1 (PD-1) on the surface. The surface expression of PD-1 is validated by FACS analysis.
M00572
¥1,613,997.00

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Overview
Product Description Recombinant CHO-K1 cells stably overexpress cynomolgus programmed cell death protein 1 (PD-1) on the surface. The surface expression of PD-1 is validated by FACS analysis.
Applications Binding assay or use as immunogen

Product Introduction
Expressed Gene NCBI reference sequence NM_001284136; no expressed tags
Target Protein NP_001271065.1
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 15 passages without significant changes in assay performance or expression profile.

Culture Conditions
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

Background
Gene Synonyms PDCD1
Background Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279), is a protein that in humans is encoded by the PDCD1 gene. PD-1, functioning as an immune checkpoint, plays an important role in down regulating the immune system by preventing the activation of T-cells, which in turn reduces autoimmunity and promotes self-tolerance. The inhibitory effect of PD-1 is accomplished through a dual mechanism of promoting apoptosis (programmed cell death) in antigen specific T-cells in lymph nodes while simultaneously reducing apoptosis in regulatory T cells (suppressor T cells)

Examples
  • CHO-K1/cyno PD-1 Stable Cell Line
    • CHO-K1/cyno PD-1 Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of cynomolgus PD-1 on CHO-K1/cyno PD-1 cells. The CHO-K1/cyno PD-1 cells (red) and the negative control CHO-K1 cells (blue) were probed using APC-conjugated anti-human CD279 antibody (Cat. No. 329908, Biolegend).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.